Comparison of AAV2 and AAV5 in gene transfer in the injured spinal cord of mice.
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Comparison of AAV2 and AAV5 in gene transfer in the injured spinal cord of mice. / Peng, Su-Ping; Kügler, Sebastian; Ma, Zhi-Kui; Shen, Yan-Qin; Schachner, Melitta.
In: NEUROREPORT, Vol. 22, No. 12, 12, 2011, p. 565-569.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Comparison of AAV2 and AAV5 in gene transfer in the injured spinal cord of mice.
AU - Peng, Su-Ping
AU - Kügler, Sebastian
AU - Ma, Zhi-Kui
AU - Shen, Yan-Qin
AU - Schachner, Melitta
PY - 2011
Y1 - 2011
N2 - Recombinant adeno-associated virus (AAV) vectors are promising tools for gene therapy. In spinal cord injury where extensive damage occurs, vectors with high diffusion and transduction abilities are required. We compared the diffusion capacity and transduction efficiency of AAV2 and AAV5 vectors using a mouse spinal cord injury model. Our study demonstrates that AAV5 is more effective than AAV2 for delivering genes into the injured spinal cord tissue. AAV5 diffused 6.9 mm from the injection site, transduced with an approximately two-fold increase in total cell number and yielded an approximately three-fold increase in gene expression in comparison with AAV2.
AB - Recombinant adeno-associated virus (AAV) vectors are promising tools for gene therapy. In spinal cord injury where extensive damage occurs, vectors with high diffusion and transduction abilities are required. We compared the diffusion capacity and transduction efficiency of AAV2 and AAV5 vectors using a mouse spinal cord injury model. Our study demonstrates that AAV5 is more effective than AAV2 for delivering genes into the injured spinal cord tissue. AAV5 diffused 6.9 mm from the injection site, transduced with an approximately two-fold increase in total cell number and yielded an approximately three-fold increase in gene expression in comparison with AAV2.
KW - Animals
KW - Female
KW - Mice
KW - Mice, Inbred C57BL
KW - Transduction, Genetic
KW - Dependovirus/genetics
KW - Gene Therapy/methods
KW - Genetic Vectors/genetics/therapeutic use
KW - Spinal Cord Injuries/therapy
KW - Transgenes
KW - Animals
KW - Female
KW - Mice
KW - Mice, Inbred C57BL
KW - Transduction, Genetic
KW - Dependovirus/genetics
KW - Gene Therapy/methods
KW - Genetic Vectors/genetics/therapeutic use
KW - Spinal Cord Injuries/therapy
KW - Transgenes
M3 - SCORING: Journal article
VL - 22
SP - 565
EP - 569
JO - NEUROREPORT
JF - NEUROREPORT
SN - 0959-4965
IS - 12
M1 - 12
ER -