Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells.
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Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. / Gagliani, Nicola; Magnani, Chiara F; Huber, Samuel; Gianolini, Monica E; Pala, Mauro; Licona-Limon, Paula; Guo, Binggege; Herbert, De'broski R; Bulfone, Alessandro; Trentini, Filippo; Clelia, Di Serio; Bacchetta, Rosa; Andreani, Marco; Brockmann, Leonie; Gregori, Silvia; Flavell, Richard A; Roncarolo, Maria-Grazia.
In: NAT MED, Vol. 19, No. 6, 6, 2013, p. 739-746.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells.
AU - Gagliani, Nicola
AU - Magnani, Chiara F
AU - Huber, Samuel
AU - Gianolini, Monica E
AU - Pala, Mauro
AU - Licona-Limon, Paula
AU - Guo, Binggege
AU - Herbert, De'broski R
AU - Bulfone, Alessandro
AU - Trentini, Filippo
AU - Clelia, Di Serio
AU - Bacchetta, Rosa
AU - Andreani, Marco
AU - Brockmann, Leonie
AU - Gregori, Silvia
AU - Flavell, Richard A
AU - Roncarolo, Maria-Grazia
PY - 2013
Y1 - 2013
N2 - CD4(+) type 1 T regulatory (Tr1) cells are induced in the periphery and have a pivotal role in promoting and maintaining tolerance. The absence of surface markers that uniquely identify Tr1 cells has limited their study and clinical applications. By gene expression profiling of human Tr1 cell clones, we identified the surface markers CD49b and lymphocyte activation gene 3 (LAG-3) as being stably and selectively coexpressed on mouse and human Tr1 cells. We showed the specificity of these markers in mouse models of intestinal inflammation and helminth infection and in the peripheral blood of healthy volunteers. The coexpression of CD49b and LAG-3 enables the isolation of highly suppressive human Tr1 cells from in vitro anergized cultures and allows the tracking of Tr1 cells in the peripheral blood of subjects who developed tolerance after allogeneic hematopoietic stem cell transplantation. The use of these markers makes it feasible to track Tr1 cells in vivo and purify Tr1 cells for cell therapy to induce or restore tolerance in subjects with immune-mediated diseases.
AB - CD4(+) type 1 T regulatory (Tr1) cells are induced in the periphery and have a pivotal role in promoting and maintaining tolerance. The absence of surface markers that uniquely identify Tr1 cells has limited their study and clinical applications. By gene expression profiling of human Tr1 cell clones, we identified the surface markers CD49b and lymphocyte activation gene 3 (LAG-3) as being stably and selectively coexpressed on mouse and human Tr1 cells. We showed the specificity of these markers in mouse models of intestinal inflammation and helminth infection and in the peripheral blood of healthy volunteers. The coexpression of CD49b and LAG-3 enables the isolation of highly suppressive human Tr1 cells from in vitro anergized cultures and allows the tracking of Tr1 cells in the peripheral blood of subjects who developed tolerance after allogeneic hematopoietic stem cell transplantation. The use of these markers makes it feasible to track Tr1 cells in vivo and purify Tr1 cells for cell therapy to induce or restore tolerance in subjects with immune-mediated diseases.
M3 - SCORING: Journal article
VL - 19
SP - 739
EP - 746
JO - NAT MED
JF - NAT MED
SN - 1078-8956
IS - 6
M1 - 6
ER -