Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease
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Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease. / Steinmeyer, K; Schwappach, B; Bens, M; Vandewalle, A; Jentsch, T J.
In: J BIOL CHEM, Vol. 270, No. 52, 29.12.1995, p. 31172-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease
AU - Steinmeyer, K
AU - Schwappach, B
AU - Bens, M
AU - Vandewalle, A
AU - Jentsch, T J
PY - 1995/12/29
Y1 - 1995/12/29
N2 - We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.
AB - We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.
KW - Animals
KW - Base Sequence
KW - Chloride Channels/genetics
KW - Cloning, Molecular
KW - Cyclic AMP/metabolism
KW - Cyclic AMP-Dependent Protein Kinases/metabolism
KW - DNA Primers
KW - DNA, Complementary
KW - Kidney Diseases/genetics
KW - Molecular Sequence Data
KW - Phosphorylation
KW - Rats
KW - Sequence Homology, Amino Acid
KW - Xenopus
U2 - 10.1074/jbc.270.52.31172
DO - 10.1074/jbc.270.52.31172
M3 - SCORING: Journal article
C2 - 8537381
VL - 270
SP - 31172
EP - 31177
JO - J BIOL CHEM
JF - J BIOL CHEM
SN - 0021-9258
IS - 52
ER -