Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease

K Steinmeyer; B Schwappach; M Bens; A Vandewalle; T J Jentsch

doi:10.1074/jbc.270.52.31172

Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease

Standard

Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease. / Steinmeyer, K; Schwappach, B; Bens, M; Vandewalle, A; Jentsch, T J.

in: J BIOL CHEM, Jahrgang 270, Nr. 52, 29.12.1995, S. 31172-7.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Steinmeyer, K, Schwappach, B, Bens, M, Vandewalle, A & Jentsch, TJ 1995, 'Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease', J BIOL CHEM, Jg. 270, Nr. 52, S. 31172-7. https://doi.org/10.1074/jbc.270.52.31172

APA

Steinmeyer, K., Schwappach, B., Bens, M., Vandewalle, A., & Jentsch, T. J. (1995). Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease. J BIOL CHEM, 270(52), 31172-7. https://doi.org/10.1074/jbc.270.52.31172

Vancouver

Steinmeyer K, Schwappach B, Bens M, Vandewalle A, Jentsch TJ. Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease. J BIOL CHEM. 1995 Dez 29;270(52):31172-7. https://doi.org/10.1074/jbc.270.52.31172

Bibtex

@article{a4ad58a4aadf4137b71bd16e364f60b5,

title = "Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease",

abstract = "We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.",

keywords = "Animals, Base Sequence, Chloride Channels/genetics, Cloning, Molecular, Cyclic AMP/metabolism, Cyclic AMP-Dependent Protein Kinases/metabolism, DNA Primers, DNA, Complementary, Kidney Diseases/genetics, Molecular Sequence Data, Phosphorylation, Rats, Sequence Homology, Amino Acid, Xenopus",

author = "K Steinmeyer and B Schwappach and M Bens and A Vandewalle and Jentsch, {T J}",

year = "1995",

month = dec,

day = "29",

doi = "10.1074/jbc.270.52.31172",

language = "English",

volume = "270",

pages = "31172--7",

journal = "J BIOL CHEM",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "52",

}

RIS

TY - JOUR

T1 - Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease

AU - Steinmeyer, K

AU - Schwappach, B

AU - Bens, M

AU - Vandewalle, A

AU - Jentsch, T J

PY - 1995/12/29

Y1 - 1995/12/29

N2 - We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.

AB - We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- > glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.

KW - Animals

KW - Base Sequence

KW - Chloride Channels/genetics

KW - Cloning, Molecular

KW - Cyclic AMP/metabolism

KW - Cyclic AMP-Dependent Protein Kinases/metabolism

KW - DNA Primers

KW - DNA, Complementary

KW - Kidney Diseases/genetics

KW - Molecular Sequence Data

KW - Phosphorylation

KW - Rats

KW - Sequence Homology, Amino Acid

KW - Xenopus

U2 - 10.1074/jbc.270.52.31172

DO - 10.1074/jbc.270.52.31172

M3 - SCORING: Journal article

C2 - 8537381

VL - 270

SP - 31172

EP - 31177

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 52

ER -