Research ExplorerPublicationsCloning and characterization of heat-inducible promoters of ...

Cloning and characterization of heat-inducible promoters of Bacillus subtilis.

Standard

Cloning and characterization of heat-inducible promoters of Bacillus subtilis. / Völker, U; Riethdorf, Sabine; Winkler, A; Weigend, B; Fortnagel, P; Hecker, M.

In: FEMS MICROBIOL LETT, Vol. 106, No. 3, 3, 1993, p. 287-293.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Völker, U, Riethdorf, S, Winkler, A, Weigend, B, Fortnagel, P & Hecker, M 1993, 'Cloning and characterization of heat-inducible promoters of Bacillus subtilis.', FEMS MICROBIOL LETT, vol. 106, no. 3, 3, pp. 287-293. <http://www.ncbi.nlm.nih.gov/pubmed/8454194?dopt=Citation>

APA

Völker, U., Riethdorf, S., Winkler, A., Weigend, B., Fortnagel, P., & Hecker, M. (1993). Cloning and characterization of heat-inducible promoters of Bacillus subtilis. FEMS MICROBIOL LETT, 106(3), 287-293. [3]. http://www.ncbi.nlm.nih.gov/pubmed/8454194?dopt=Citation

Vancouver

Völker U, Riethdorf S, Winkler A, Weigend B, Fortnagel P, Hecker M. Cloning and characterization of heat-inducible promoters of Bacillus subtilis. FEMS MICROBIOL LETT. 1993;106(3):287-293. 3.

Bibtex

@article{19579e5c188840378e40d130407e59b3,
title = "Cloning and characterization of heat-inducible promoters of Bacillus subtilis.",
abstract = "Heat-inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structure of the heat-sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.",
keywords = "Molecular Sequence Data, Base Sequence, Transcription, Genetic, Genes, Bacterial, Chromosome Mapping, Hot Temperature, Cloning, Molecular, Genetic Vectors, *Promoter Regions, Genetic, Bacillus subtilis/*genetics, Catechol 2,3-Dioxygenase, DNA Probes, DNA, Bacterial/genetics, *Dioxygenases, Glycoside Hydrolases/genetics, Heat-Shock Proteins/genetics, Oxygenases/genetics, Sigma Factor/genetics, Molecular Sequence Data, Base Sequence, Transcription, Genetic, Genes, Bacterial, Chromosome Mapping, Hot Temperature, Cloning, Molecular, Genetic Vectors, *Promoter Regions, Genetic, Bacillus subtilis/*genetics, Catechol 2,3-Dioxygenase, DNA Probes, DNA, Bacterial/genetics, *Dioxygenases, Glycoside Hydrolases/genetics, Heat-Shock Proteins/genetics, Oxygenases/genetics, Sigma Factor/genetics",
author = "U V{\"o}lker and Sabine Riethdorf and A Winkler and B Weigend and P Fortnagel and M Hecker",
year = "1993",
language = "English",
volume = "106",
pages = "287--293",
journal = "FEMS MICROBIOL LETT",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Cloning and characterization of heat-inducible promoters of Bacillus subtilis.

AU - Völker, U

AU - Riethdorf, Sabine

AU - Winkler, A

AU - Weigend, B

AU - Fortnagel, P

AU - Hecker, M

PY - 1993

Y1 - 1993

N2 - Heat-inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structure of the heat-sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.

AB - Heat-inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structure of the heat-sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.

KW - Molecular Sequence Data

KW - Base Sequence

KW - Transcription, Genetic

KW - Genes, Bacterial

KW - Chromosome Mapping

KW - Hot Temperature

KW - Cloning, Molecular

KW - Genetic Vectors

KW - Promoter Regions, Genetic

KW - Bacillus subtilis/genetics

KW - Catechol 2,3-Dioxygenase

KW - DNA Probes

KW - DNA, Bacterial/genetics

KW - Dioxygenases

KW - Glycoside Hydrolases/genetics

KW - Heat-Shock Proteins/genetics

KW - Oxygenases/genetics

KW - Sigma Factor/genetics

KW - Molecular Sequence Data

KW - Base Sequence

KW - Transcription, Genetic

KW - Genes, Bacterial

KW - Chromosome Mapping

KW - Hot Temperature

KW - Cloning, Molecular

KW - Genetic Vectors

KW - Promoter Regions, Genetic

KW - Bacillus subtilis/genetics

KW - Catechol 2,3-Dioxygenase

KW - DNA Probes

KW - DNA, Bacterial/genetics

KW - Dioxygenases

KW - Glycoside Hydrolases/genetics

KW - Heat-Shock Proteins/genetics

KW - Oxygenases/genetics

KW - Sigma Factor/genetics

M3 - SCORING: Journal article

VL - 106

SP - 287

EP - 293

JO - FEMS MICROBIOL LETT

JF - FEMS MICROBIOL LETT

SN - 0378-1097

IS - 3

M1 - 3

ER -