Cloning and characterization of heat-inducible promoters of Bacillus subtilis.
Standard
Cloning and characterization of heat-inducible promoters of Bacillus subtilis. / Völker, U; Riethdorf, Sabine; Winkler, A; Weigend, B; Fortnagel, P; Hecker, M.
in: FEMS MICROBIOL LETT, Jahrgang 106, Nr. 3, 3, 1993, S. 287-293.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Cloning and characterization of heat-inducible promoters of Bacillus subtilis.
AU - Völker, U
AU - Riethdorf, Sabine
AU - Winkler, A
AU - Weigend, B
AU - Fortnagel, P
AU - Hecker, M
PY - 1993
Y1 - 1993
N2 - Heat-inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structure of the heat-sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.
AB - Heat-inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structure of the heat-sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis. Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli, where alternative sigma factors direct the transcription of heat shock genes.
KW - Molecular Sequence Data
KW - Base Sequence
KW - Transcription, Genetic
KW - Genes, Bacterial
KW - Chromosome Mapping
KW - Hot Temperature
KW - Cloning, Molecular
KW - Genetic Vectors
KW - Promoter Regions, Genetic
KW - Bacillus subtilis/genetics
KW - Catechol 2,3-Dioxygenase
KW - DNA Probes
KW - DNA, Bacterial/genetics
KW - Dioxygenases
KW - Glycoside Hydrolases/genetics
KW - Heat-Shock Proteins/genetics
KW - Oxygenases/genetics
KW - Sigma Factor/genetics
KW - Molecular Sequence Data
KW - Base Sequence
KW - Transcription, Genetic
KW - Genes, Bacterial
KW - Chromosome Mapping
KW - Hot Temperature
KW - Cloning, Molecular
KW - Genetic Vectors
KW - Promoter Regions, Genetic
KW - Bacillus subtilis/genetics
KW - Catechol 2,3-Dioxygenase
KW - DNA Probes
KW - DNA, Bacterial/genetics
KW - Dioxygenases
KW - Glycoside Hydrolases/genetics
KW - Heat-Shock Proteins/genetics
KW - Oxygenases/genetics
KW - Sigma Factor/genetics
M3 - SCORING: Journal article
VL - 106
SP - 287
EP - 293
JO - FEMS MICROBIOL LETT
JF - FEMS MICROBIOL LETT
SN - 0378-1097
IS - 3
M1 - 3
ER -