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Clinical relevance of cytoskeleton associated proteins for ovarian cancer

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Clinical relevance of cytoskeleton associated proteins for ovarian cancer. / Schiewek, Johanna; Schumacher, Udo; Lange, Tobias; Joosse, Simon A; Wikman, Harriet; Pantel, Klaus; Mikhaylova, Marina; Kneussel, Matthias; Linder, Stefan; Schmalfeldt, Barbara; Oliveira-Ferrer, Leticia; Windhorst, Sabine.

In: J CANCER RES CLIN, Vol. 144, No. 11, 11.2018, p. 2195-2205.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schiewek, J, Schumacher, U, Lange, T, Joosse, SA, Wikman, H, Pantel, K, Mikhaylova, M, Kneussel, M, Linder, S, Schmalfeldt, B, Oliveira-Ferrer, L & Windhorst, S 2018, 'Clinical relevance of cytoskeleton associated proteins for ovarian cancer', J CANCER RES CLIN, vol. 144, no. 11, pp. 2195-2205. https://doi.org/10.1007/s00432-018-2710-9

APA

Schiewek, J., Schumacher, U., Lange, T., Joosse, S. A., Wikman, H., Pantel, K., Mikhaylova, M., Kneussel, M., Linder, S., Schmalfeldt, B., Oliveira-Ferrer, L., & Windhorst, S. (2018). Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J CANCER RES CLIN, 144(11), 2195-2205. https://doi.org/10.1007/s00432-018-2710-9

Vancouver

Schiewek J, Schumacher U, Lange T, Joosse SA, Wikman H, Pantel K et al. Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J CANCER RES CLIN. 2018 Nov;144(11):2195-2205. https://doi.org/10.1007/s00432-018-2710-9

Bibtex

@article{f81a62875b17444883803c00dc6c6fde,
title = "Clinical relevance of cytoskeleton associated proteins for ovarian cancer",
abstract = "PURPOSE: Ovarian cancer has a high mortality rate and up to now no reliable molecular prognostic biomarkers have been established. During malignant progression, the cytoskeleton is strongly altered. Hence we analyzed if expression of certain cytoskeleton-associated proteins is correlated with clinical outcome of ovarian cancer patients.METHODS: First, in silico analysis was performed using the cancer genome atlas (TCGA), the human expression atlas and Pubmed. Selected candidates were validated on 270 ovarian cancer patients by qRT-PCR and/or by western blotting.RESULTS: In silico analysis revealed that mRNAs of 214 cytoskeleton-associated proteins are detectable in ovarian cancer tissue. Among these, we selected 17 proteins that participate in cancer disease progression and cytoskeleton modulation: KIF14, KIF20A, KIF18A, ASPM, CEP55, DLGAP5, MAP9, EB1, KATNA1, DIAPH1, ANLN, SCIN, CCDC88A, FSCN1, GSN, VASP and CDC42. The first ten candidates interact with microtubules (MTs) and the others bind to actin filaments. Validation on clinical samples of ovarian cancer patients revealed that the expression levels of DIAPH1, EB1, KATNA1, KIF14 and KIF18A significantly correlated with clinical and histological parameters of ovarian cancer. High DIAPH1, EB1, KATNA1 and KIF14 protein levels were associated with increased overall survival (OAS) of ovarian cancer patients, while high DIAPH1 and EB1 protein levels were also associated with low differentiation of respective tumors (G2/3). Moreover, DIAPH1 was the only protein, whose expression significantly correlated with increased recurrence-free interval (RFI).CONCLUSION: Mainly the expression levels of the MT-associated proteins analyzed in this study, correlated with prolonged survival of ovarian cancer patients. From > 200 genes initially considered, 17 cytoskeletal proteins are involved in cancer progression according to the literature. Among these, four proteins significantly correlated with improved survival of ovarian cancer patients.",
keywords = "Journal Article",
author = "Johanna Schiewek and Udo Schumacher and Tobias Lange and Joosse, {Simon A} and Harriet Wikman and Klaus Pantel and Marina Mikhaylova and Matthias Kneussel and Stefan Linder and Barbara Schmalfeldt and Leticia Oliveira-Ferrer and Sabine Windhorst",
year = "2018",
month = nov,
doi = "10.1007/s00432-018-2710-9",
language = "English",
volume = "144",
pages = "2195--2205",
journal = "J CANCER RES CLIN",
issn = "0171-5216",
publisher = "Springer",
number = "11",

}

RIS

TY - JOUR

T1 - Clinical relevance of cytoskeleton associated proteins for ovarian cancer

AU - Schiewek, Johanna

AU - Schumacher, Udo

AU - Lange, Tobias

AU - Joosse, Simon A

AU - Wikman, Harriet

AU - Pantel, Klaus

AU - Mikhaylova, Marina

AU - Kneussel, Matthias

AU - Linder, Stefan

AU - Schmalfeldt, Barbara

AU - Oliveira-Ferrer, Leticia

AU - Windhorst, Sabine

PY - 2018/11

Y1 - 2018/11

N2 - PURPOSE: Ovarian cancer has a high mortality rate and up to now no reliable molecular prognostic biomarkers have been established. During malignant progression, the cytoskeleton is strongly altered. Hence we analyzed if expression of certain cytoskeleton-associated proteins is correlated with clinical outcome of ovarian cancer patients.METHODS: First, in silico analysis was performed using the cancer genome atlas (TCGA), the human expression atlas and Pubmed. Selected candidates were validated on 270 ovarian cancer patients by qRT-PCR and/or by western blotting.RESULTS: In silico analysis revealed that mRNAs of 214 cytoskeleton-associated proteins are detectable in ovarian cancer tissue. Among these, we selected 17 proteins that participate in cancer disease progression and cytoskeleton modulation: KIF14, KIF20A, KIF18A, ASPM, CEP55, DLGAP5, MAP9, EB1, KATNA1, DIAPH1, ANLN, SCIN, CCDC88A, FSCN1, GSN, VASP and CDC42. The first ten candidates interact with microtubules (MTs) and the others bind to actin filaments. Validation on clinical samples of ovarian cancer patients revealed that the expression levels of DIAPH1, EB1, KATNA1, KIF14 and KIF18A significantly correlated with clinical and histological parameters of ovarian cancer. High DIAPH1, EB1, KATNA1 and KIF14 protein levels were associated with increased overall survival (OAS) of ovarian cancer patients, while high DIAPH1 and EB1 protein levels were also associated with low differentiation of respective tumors (G2/3). Moreover, DIAPH1 was the only protein, whose expression significantly correlated with increased recurrence-free interval (RFI).CONCLUSION: Mainly the expression levels of the MT-associated proteins analyzed in this study, correlated with prolonged survival of ovarian cancer patients. From > 200 genes initially considered, 17 cytoskeletal proteins are involved in cancer progression according to the literature. Among these, four proteins significantly correlated with improved survival of ovarian cancer patients.

AB - PURPOSE: Ovarian cancer has a high mortality rate and up to now no reliable molecular prognostic biomarkers have been established. During malignant progression, the cytoskeleton is strongly altered. Hence we analyzed if expression of certain cytoskeleton-associated proteins is correlated with clinical outcome of ovarian cancer patients.METHODS: First, in silico analysis was performed using the cancer genome atlas (TCGA), the human expression atlas and Pubmed. Selected candidates were validated on 270 ovarian cancer patients by qRT-PCR and/or by western blotting.RESULTS: In silico analysis revealed that mRNAs of 214 cytoskeleton-associated proteins are detectable in ovarian cancer tissue. Among these, we selected 17 proteins that participate in cancer disease progression and cytoskeleton modulation: KIF14, KIF20A, KIF18A, ASPM, CEP55, DLGAP5, MAP9, EB1, KATNA1, DIAPH1, ANLN, SCIN, CCDC88A, FSCN1, GSN, VASP and CDC42. The first ten candidates interact with microtubules (MTs) and the others bind to actin filaments. Validation on clinical samples of ovarian cancer patients revealed that the expression levels of DIAPH1, EB1, KATNA1, KIF14 and KIF18A significantly correlated with clinical and histological parameters of ovarian cancer. High DIAPH1, EB1, KATNA1 and KIF14 protein levels were associated with increased overall survival (OAS) of ovarian cancer patients, while high DIAPH1 and EB1 protein levels were also associated with low differentiation of respective tumors (G2/3). Moreover, DIAPH1 was the only protein, whose expression significantly correlated with increased recurrence-free interval (RFI).CONCLUSION: Mainly the expression levels of the MT-associated proteins analyzed in this study, correlated with prolonged survival of ovarian cancer patients. From > 200 genes initially considered, 17 cytoskeletal proteins are involved in cancer progression according to the literature. Among these, four proteins significantly correlated with improved survival of ovarian cancer patients.

KW - Journal Article

U2 - 10.1007/s00432-018-2710-9

DO - 10.1007/s00432-018-2710-9

M3 - SCORING: Journal article

C2 - 30094535

VL - 144

SP - 2195

EP - 2205

JO - J CANCER RES CLIN

JF - J CANCER RES CLIN

SN - 0171-5216

IS - 11

ER -