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Characterization of KIBRA, a novel WW domain-containing protein

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Characterization of KIBRA, a novel WW domain-containing protein. / Kremerskothen, Joachim; Plaas, Christian; Büther, Katrin; Finger, Indra; Veltel, Stefan; Matanis, Theodoros; Liedtke, Thomas; Barnekow, Angelika.

In: BIOCHEM BIOPH RES CO, Vol. 300, No. 4, 24.01.2003, p. 862-7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kremerskothen, J, Plaas, C, Büther, K, Finger, I, Veltel, S, Matanis, T, Liedtke, T & Barnekow, A 2003, 'Characterization of KIBRA, a novel WW domain-containing protein', BIOCHEM BIOPH RES CO, vol. 300, no. 4, pp. 862-7.

APA

Kremerskothen, J., Plaas, C., Büther, K., Finger, I., Veltel, S., Matanis, T., Liedtke, T., & Barnekow, A. (2003). Characterization of KIBRA, a novel WW domain-containing protein. BIOCHEM BIOPH RES CO, 300(4), 862-7.

Vancouver

Kremerskothen J, Plaas C, Büther K, Finger I, Veltel S, Matanis T et al. Characterization of KIBRA, a novel WW domain-containing protein. BIOCHEM BIOPH RES CO. 2003 Jan 24;300(4):862-7.

Bibtex

@article{c4d102dca1c347d3baefead08721dcbb,
title = "Characterization of KIBRA, a novel WW domain-containing protein",
abstract = "In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.",
keywords = "Amino Acid Sequence, Animals, Cells, Cultured, Cercopithecus aethiops, Humans, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Mutagenesis, Site-Directed, Nerve Tissue Proteins, Phosphoproteins, Protein Structure, Tertiary, Proteins, Recombinant Fusion Proteins, Sequence Alignment, Two-Hybrid System Techniques",
author = "Joachim Kremerskothen and Christian Plaas and Katrin B{\"u}ther and Indra Finger and Stefan Veltel and Theodoros Matanis and Thomas Liedtke and Angelika Barnekow",
year = "2003",
month = jan,
day = "24",
language = "English",
volume = "300",
pages = "862--7",
journal = "BIOCHEM BIOPH RES CO",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Characterization of KIBRA, a novel WW domain-containing protein

AU - Kremerskothen, Joachim

AU - Plaas, Christian

AU - Büther, Katrin

AU - Finger, Indra

AU - Veltel, Stefan

AU - Matanis, Theodoros

AU - Liedtke, Thomas

AU - Barnekow, Angelika

PY - 2003/1/24

Y1 - 2003/1/24

N2 - In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.

AB - In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.

KW - Amino Acid Sequence

KW - Animals

KW - Cells, Cultured

KW - Cercopithecus aethiops

KW - Humans

KW - Intracellular Signaling Peptides and Proteins

KW - Molecular Sequence Data

KW - Mutagenesis, Site-Directed

KW - Nerve Tissue Proteins

KW - Phosphoproteins

KW - Protein Structure, Tertiary

KW - Proteins

KW - Recombinant Fusion Proteins

KW - Sequence Alignment

KW - Two-Hybrid System Techniques

M3 - SCORING: Journal article

C2 - 12559952

VL - 300

SP - 862

EP - 867

JO - BIOCHEM BIOPH RES CO

JF - BIOCHEM BIOPH RES CO

SN - 0006-291X

IS - 4

ER -