Characterization of KIBRA, a novel WW domain-containing protein
Standard
Characterization of KIBRA, a novel WW domain-containing protein. / Kremerskothen, Joachim; Plaas, Christian; Büther, Katrin; Finger, Indra; Veltel, Stefan; Matanis, Theodoros; Liedtke, Thomas; Barnekow, Angelika.
in: BIOCHEM BIOPH RES CO, Jahrgang 300, Nr. 4, 24.01.2003, S. 862-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Characterization of KIBRA, a novel WW domain-containing protein
AU - Kremerskothen, Joachim
AU - Plaas, Christian
AU - Büther, Katrin
AU - Finger, Indra
AU - Veltel, Stefan
AU - Matanis, Theodoros
AU - Liedtke, Thomas
AU - Barnekow, Angelika
PY - 2003/1/24
Y1 - 2003/1/24
N2 - In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.
AB - In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.
KW - Amino Acid Sequence
KW - Animals
KW - Cells, Cultured
KW - Cercopithecus aethiops
KW - Humans
KW - Intracellular Signaling Peptides and Proteins
KW - Molecular Sequence Data
KW - Mutagenesis, Site-Directed
KW - Nerve Tissue Proteins
KW - Phosphoproteins
KW - Protein Structure, Tertiary
KW - Proteins
KW - Recombinant Fusion Proteins
KW - Sequence Alignment
KW - Two-Hybrid System Techniques
M3 - SCORING: Journal article
C2 - 12559952
VL - 300
SP - 862
EP - 867
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 4
ER -