Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).
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Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1). / Hayashi, M; Fujimoto, S; Takano, H; Ushiki, T; Abe, K; Ishikura, H; Yoshida, M C; Kirchhoff, C; Ishibashi, T; Kasahara, M.
In: GENOMICS, Vol. 32, No. 3, 3, 15.03.1996, p. 367-374.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).
AU - Hayashi, M
AU - Fujimoto, S
AU - Takano, H
AU - Ushiki, T
AU - Abe, K
AU - Ishikura, H
AU - Yoshida, M C
AU - Kirchhoff, C
AU - Ishibashi, T
AU - Kasahara, M
PY - 1996/3/15
Y1 - 1996/3/15
N2 - Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head. The fact that no AEG mRNA can be detected in the human epididymis suggests that ARP might be the functional counterpart of rodent AEG. The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21.2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies.
AB - Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head. The fact that no AEG mRNA can be detected in the human epididymis suggests that ARP might be the functional counterpart of rodent AEG. The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21.2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies.
KW - Acrosome
KW - Aged
KW - Amino Acid Sequence
KW - Animals
KW - Base Sequence
KW - Chromosome Mapping
KW - Cloning, Molecular
KW - DNA, Complementary
KW - Epididymis
KW - Female
KW - Genes
KW - Humans
KW - Male
KW - Membrane Glycoproteins
KW - Middle Aged
KW - Molecular Sequence Data
KW - Molecular Weight
KW - RNA, Messenger
KW - Sequence Alignment
KW - Sequence Analysis, DNA
KW - Sequence Homology, Nucleic Acid
KW - Species Specificity
KW - Sperm-Ovum Interactions
KW - Testis
U2 - 10.1006/geno.1996.0131
DO - 10.1006/geno.1996.0131
M3 - SCORING: Journal article
C2 - 8838800
VL - 32
SP - 367
EP - 374
IS - 3
M1 - 3
ER -