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Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).

Standard

Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1). / Hayashi, M; Fujimoto, S; Takano, H; Ushiki, T; Abe, K; Ishikura, H; Yoshida, M C; Kirchhoff, C; Ishibashi, T; Kasahara, M.

in: GENOMICS, Jahrgang 32, Nr. 3, 3, 15.03.1996, S. 367-374.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hayashi, M, Fujimoto, S, Takano, H, Ushiki, T, Abe, K, Ishikura, H, Yoshida, MC, Kirchhoff, C, Ishibashi, T & Kasahara, M 1996, 'Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).', GENOMICS, Jg. 32, Nr. 3, 3, S. 367-374. https://doi.org/10.1006/geno.1996.0131

APA

Hayashi, M., Fujimoto, S., Takano, H., Ushiki, T., Abe, K., Ishikura, H., Yoshida, M. C., Kirchhoff, C., Ishibashi, T., & Kasahara, M. (1996). Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1). GENOMICS, 32(3), 367-374. [3]. https://doi.org/10.1006/geno.1996.0131

Vancouver

Hayashi M, Fujimoto S, Takano H, Ushiki T, Abe K, Ishikura H et al. Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1). GENOMICS. 1996 Mär 15;32(3):367-374. 3. https://doi.org/10.1006/geno.1996.0131

Bibtex

@article{ed902e1204444f0c99b1dc9656fe3fea,
title = "Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).",
abstract = "Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head. The fact that no AEG mRNA can be detected in the human epididymis suggests that ARP might be the functional counterpart of rodent AEG. The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21.2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies.",
keywords = "Acrosome, Aged, Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, DNA, Complementary, Epididymis, Female, Genes, Humans, Male, Membrane Glycoproteins, Middle Aged, Molecular Sequence Data, Molecular Weight, RNA, Messenger, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Species Specificity, Sperm-Ovum Interactions, Testis",
author = "M Hayashi and S Fujimoto and H Takano and T Ushiki and K Abe and H Ishikura and Yoshida, {M C} and C Kirchhoff and T Ishibashi and M Kasahara",
year = "1996",
month = mar,
day = "15",
doi = "10.1006/geno.1996.0131",
language = "English",
volume = "32",
pages = "367--374",
number = "3",

}

RIS

TY - JOUR

T1 - Characterization of a human glycoprotein with a potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1).

AU - Hayashi, M

AU - Fujimoto, S

AU - Takano, H

AU - Ushiki, T

AU - Abe, K

AU - Ishikura, H

AU - Yoshida, M C

AU - Kirchhoff, C

AU - Ishibashi, T

AU - Kasahara, M

PY - 1996/3/15

Y1 - 1996/3/15

N2 - Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head. The fact that no AEG mRNA can be detected in the human epididymis suggests that ARP might be the functional counterpart of rodent AEG. The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21.2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies.

AB - Acidic epididymal glycoprotein (AEG), thus far identified only in rodents, is one of the sperm surface proteins involved in the fusion of the sperm and egg plasma membranes. In the present study, we describe the isolation and characterization of cDNA encoding a human glycoprotein related to AEG. Although this protein, designated ARP (AEG-related protein), is not the ortholog of rodent AEG, it resembles AEG in that it is an epididymal secretory glycoprotein that binds to the postacrosomal region of the sperm head. The fact that no AEG mRNA can be detected in the human epididymis suggests that ARP might be the functional counterpart of rodent AEG. The gene encoding ARP (AEGL1) was mapped by fluorescence in situ hybridization to 6p21.1-p21.2. This result indicates that AEGL1 and the mouse gene for AEG are located in the chromosomal segments with conserved syntenies.

KW - Acrosome

KW - Aged

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Chromosome Mapping

KW - Cloning, Molecular

KW - DNA, Complementary

KW - Epididymis

KW - Female

KW - Genes

KW - Humans

KW - Male

KW - Membrane Glycoproteins

KW - Middle Aged

KW - Molecular Sequence Data

KW - Molecular Weight

KW - RNA, Messenger

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Sequence Homology, Nucleic Acid

KW - Species Specificity

KW - Sperm-Ovum Interactions

KW - Testis

U2 - 10.1006/geno.1996.0131

DO - 10.1006/geno.1996.0131

M3 - SCORING: Journal article

C2 - 8838800

VL - 32

SP - 367

EP - 374

IS - 3

M1 - 3

ER -