Changes of acetylcholinesterase molecular forms in regenerating motor neurons
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Changes of acetylcholinesterase molecular forms in regenerating motor neurons. / Engel, A K; Kreutzberg, G W.
In: NEUROSCIENCE, Vol. 18, No. 2, 01.06.1986, p. 467-73.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Changes of acetylcholinesterase molecular forms in regenerating motor neurons
AU - Engel, A K
AU - Kreutzberg, G W
PY - 1986/6/1
Y1 - 1986/6/1
N2 - Axotomy-induced changes of the molecular forms of acetylcholinesterase in the facial nucleus of the rat and guinea pig were investigated. Evidence is presented that facial motoneurons of the guinea pig are capable of synthesizing considerable amounts of 16S acetylcholinesterase, and furthermore that acetylcholinesterase isoenzymes show species differences in their response to axon transection. Three isoenzymes could be separated by velocity sedimentation, which correspond to G1 (4S), G4 (10S) and A12 (16S) acetylcholinesterase. After axotomy, G4 activity was decreased in both species by 40% 2-3 weeks after nerve transection. In the rat, G1 was even further depressed, whereas in guinea pig facial nucleus G1 showed only a slight change. A12 displayed a clear species difference: in the rat, it was decreased to 60% of control 5 days after axotomy. In guinea pig, however, A12 increased dramatically to values of 400-500% of the unoperated control, and maintained elevated levels even 120 days after operation. This result does not agree with the decrease of transmitter metabolism in regenerating nerves and provides support to the hypothesis that acetylcholinesterase in regenerating nerves may have functions different from transmitter hydrolysis.
AB - Axotomy-induced changes of the molecular forms of acetylcholinesterase in the facial nucleus of the rat and guinea pig were investigated. Evidence is presented that facial motoneurons of the guinea pig are capable of synthesizing considerable amounts of 16S acetylcholinesterase, and furthermore that acetylcholinesterase isoenzymes show species differences in their response to axon transection. Three isoenzymes could be separated by velocity sedimentation, which correspond to G1 (4S), G4 (10S) and A12 (16S) acetylcholinesterase. After axotomy, G4 activity was decreased in both species by 40% 2-3 weeks after nerve transection. In the rat, G1 was even further depressed, whereas in guinea pig facial nucleus G1 showed only a slight change. A12 displayed a clear species difference: in the rat, it was decreased to 60% of control 5 days after axotomy. In guinea pig, however, A12 increased dramatically to values of 400-500% of the unoperated control, and maintained elevated levels even 120 days after operation. This result does not agree with the decrease of transmitter metabolism in regenerating nerves and provides support to the hypothesis that acetylcholinesterase in regenerating nerves may have functions different from transmitter hydrolysis.
KW - Acetylcholinesterase
KW - Animals
KW - Chickens
KW - Facial Nerve
KW - Guinea Pigs
KW - Isoenzymes
KW - Motor Neurons
KW - Nerve Regeneration
KW - Pons
KW - Rats
KW - Species Specificity
M3 - SCORING: Journal article
C2 - 3736865
VL - 18
SP - 467
EP - 473
JO - NEUROSCIENCE
JF - NEUROSCIENCE
SN - 0306-4522
IS - 2
ER -