Brain-derived neurotrophic factor increases neurotrophin-3 expression in cerebellar granule neurons
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Brain-derived neurotrophic factor increases neurotrophin-3 expression in cerebellar granule neurons. / Leingärtner, A; Heisenberg, C P; Kolbeck, R; Thoenen, H; Lindholm, D.
In: J BIOL CHEM, Vol. 269, No. 2, 14.01.1994, p. 828-30.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Brain-derived neurotrophic factor increases neurotrophin-3 expression in cerebellar granule neurons
AU - Leingärtner, A
AU - Heisenberg, C P
AU - Kolbeck, R
AU - Thoenen, H
AU - Lindholm, D
PY - 1994/1/14
Y1 - 1994/1/14
N2 - Neurotrophin-3 (NT-3) is a member of the neurotrophin gene family and is highly expressed in the developing rat cerebellum. Here we show that brain-derived neurotrophic factor (BDNF) increased by approximately 10-fold the NT-3 mRNA levels in cultured cerebellar granule neurons isolated from postnatal rats, whereas nerve growth factor (NGF) and NT-3 itself had no effect. The effect of BDNF was additive to that of triiodothyronine (T3), which also increased NT-3 mRNA in these neurons. The drug K252a inhibited the BDNF-mediated stimulation of NT-3 expression, suggesting an involvement of trkB receptors. Nuclear run-on experiments showed that BDNF enhanced NT-3 transcription, whereas the stability of NT-3 mRNA remained unchanged. The data presented are the first demonstration that one neurotrophin regulates the expression of another and provide evidence that NT-3 production in granule neurons is regulated by both BDNF and T3.
AB - Neurotrophin-3 (NT-3) is a member of the neurotrophin gene family and is highly expressed in the developing rat cerebellum. Here we show that brain-derived neurotrophic factor (BDNF) increased by approximately 10-fold the NT-3 mRNA levels in cultured cerebellar granule neurons isolated from postnatal rats, whereas nerve growth factor (NGF) and NT-3 itself had no effect. The effect of BDNF was additive to that of triiodothyronine (T3), which also increased NT-3 mRNA in these neurons. The drug K252a inhibited the BDNF-mediated stimulation of NT-3 expression, suggesting an involvement of trkB receptors. Nuclear run-on experiments showed that BDNF enhanced NT-3 transcription, whereas the stability of NT-3 mRNA remained unchanged. The data presented are the first demonstration that one neurotrophin regulates the expression of another and provide evidence that NT-3 production in granule neurons is regulated by both BDNF and T3.
KW - Animals
KW - Brain-Derived Neurotrophic Factor
KW - Cerebellum
KW - Gene Expression
KW - Nerve Growth Factors
KW - Nerve Tissue Proteins
KW - Neurotrophin 3
KW - RNA, Messenger
KW - Rats
KW - Transcription, Genetic
KW - Triiodothyronine
M3 - SCORING: Journal article
C2 - 8288635
VL - 269
SP - 828
EP - 830
JO - J BIOL CHEM
JF - J BIOL CHEM
SN - 0021-9258
IS - 2
ER -