Brain protein preservation largely depends on the postmortem storage temperature
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Brain protein preservation largely depends on the postmortem storage temperature : implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study. / Ferrer, Isidre; Santpere, Gabriel; Arzberger, Thomas; Bell, Jeanne; Blanco, Rosa; Boluda, Susana; Budka, Herbert; Carmona, Margarita; Giaccone, Giorgio; Krebs, Bjarne; Limido, Lucia; Parchi, Piero; Puig, Berta; Strammiello, Rosaria; Ströbel, Thomas; Kretzschmar, Hans; Puig Martorell, Berta.
In: J NEUROPATH EXP NEUR, Vol. 66, No. 1, 01.01.2007, p. 35-46.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Brain protein preservation largely depends on the postmortem storage temperature
T2 - implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study
AU - Ferrer, Isidre
AU - Santpere, Gabriel
AU - Arzberger, Thomas
AU - Bell, Jeanne
AU - Blanco, Rosa
AU - Boluda, Susana
AU - Budka, Herbert
AU - Carmona, Margarita
AU - Giaccone, Giorgio
AU - Krebs, Bjarne
AU - Limido, Lucia
AU - Parchi, Piero
AU - Puig, Berta
AU - Strammiello, Rosaria
AU - Ströbel, Thomas
AU - Kretzschmar, Hans
AU - Puig Martorell, Berta
PY - 2007/1/1
Y1 - 2007/1/1
N2 - The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1 degrees C, 4 degrees C, or room temperature and then frozen at -80 degrees C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1 degrees C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4 degrees C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.
AB - The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1 degrees C, 4 degrees C, or room temperature and then frozen at -80 degrees C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1 degrees C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4 degrees C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.
KW - Aged
KW - Alzheimer Disease
KW - Blotting, Western
KW - Brain
KW - Brain Chemistry
KW - Electrophoresis, Gel, Two-Dimensional
KW - Europe
KW - Female
KW - Humans
KW - Male
KW - Middle Aged
KW - Postmortem Changes
KW - Proteins
KW - Temperature
KW - Time Factors
KW - Tissue Preservation
KW - Tissue Survival
U2 - 10.1097/nen.0b013e31802c3e7d
DO - 10.1097/nen.0b013e31802c3e7d
M3 - SCORING: Journal article
C2 - 17204935
VL - 66
SP - 35
EP - 46
JO - J NEUROPATH EXP NEUR
JF - J NEUROPATH EXP NEUR
SN - 0022-3069
IS - 1
ER -