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Brain protein preservation largely depends on the postmortem storage temperature

Standard

Brain protein preservation largely depends on the postmortem storage temperature : implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study. / Ferrer, Isidre; Santpere, Gabriel; Arzberger, Thomas; Bell, Jeanne; Blanco, Rosa; Boluda, Susana; Budka, Herbert; Carmona, Margarita; Giaccone, Giorgio; Krebs, Bjarne; Limido, Lucia; Parchi, Piero; Puig, Berta; Strammiello, Rosaria; Ströbel, Thomas; Kretzschmar, Hans; Puig Martorell, Berta.

in: J NEUROPATH EXP NEUR, Jahrgang 66, Nr. 1, 01.01.2007, S. 35-46.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ferrer, I, Santpere, G, Arzberger, T, Bell, J, Blanco, R, Boluda, S, Budka, H, Carmona, M, Giaccone, G, Krebs, B, Limido, L, Parchi, P, Puig, B, Strammiello, R, Ströbel, T, Kretzschmar, H & Puig Martorell, B 2007, 'Brain protein preservation largely depends on the postmortem storage temperature: implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study', J NEUROPATH EXP NEUR, Jg. 66, Nr. 1, S. 35-46. https://doi.org/10.1097/nen.0b013e31802c3e7d

APA

Ferrer, I., Santpere, G., Arzberger, T., Bell, J., Blanco, R., Boluda, S., Budka, H., Carmona, M., Giaccone, G., Krebs, B., Limido, L., Parchi, P., Puig, B., Strammiello, R., Ströbel, T., Kretzschmar, H., & Puig Martorell, B. (2007). Brain protein preservation largely depends on the postmortem storage temperature: implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study. J NEUROPATH EXP NEUR, 66(1), 35-46. https://doi.org/10.1097/nen.0b013e31802c3e7d

Vancouver

Ferrer I, Santpere G, Arzberger T, Bell J, Blanco R, Boluda S et al. Brain protein preservation largely depends on the postmortem storage temperature: implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study. J NEUROPATH EXP NEUR. 2007 Jan 1;66(1):35-46. https://doi.org/10.1097/nen.0b013e31802c3e7d

Bibtex

@article{63d5d0aec8e348fa829c4659c470264a,
title = "Brain protein preservation largely depends on the postmortem storage temperature: implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study",
abstract = "The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1 degrees C, 4 degrees C, or room temperature and then frozen at -80 degrees C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1 degrees C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4 degrees C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.",
keywords = "Aged, Alzheimer Disease, Blotting, Western, Brain, Brain Chemistry, Electrophoresis, Gel, Two-Dimensional, Europe, Female, Humans, Male, Middle Aged, Postmortem Changes, Proteins, Temperature, Time Factors, Tissue Preservation, Tissue Survival",
author = "Isidre Ferrer and Gabriel Santpere and Thomas Arzberger and Jeanne Bell and Rosa Blanco and Susana Boluda and Herbert Budka and Margarita Carmona and Giorgio Giaccone and Bjarne Krebs and Lucia Limido and Piero Parchi and Berta Puig and Rosaria Strammiello and Thomas Str{\"o}bel and Hans Kretzschmar and {Puig Martorell}, Berta",
year = "2007",
month = jan,
day = "1",
doi = "10.1097/nen.0b013e31802c3e7d",
language = "English",
volume = "66",
pages = "35--46",
journal = "J NEUROPATH EXP NEUR",
issn = "0022-3069",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

RIS

TY - JOUR

T1 - Brain protein preservation largely depends on the postmortem storage temperature

T2 - implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study

AU - Ferrer, Isidre

AU - Santpere, Gabriel

AU - Arzberger, Thomas

AU - Bell, Jeanne

AU - Blanco, Rosa

AU - Boluda, Susana

AU - Budka, Herbert

AU - Carmona, Margarita

AU - Giaccone, Giorgio

AU - Krebs, Bjarne

AU - Limido, Lucia

AU - Parchi, Piero

AU - Puig, Berta

AU - Strammiello, Rosaria

AU - Ströbel, Thomas

AU - Kretzschmar, Hans

AU - Puig Martorell, Berta

PY - 2007/1/1

Y1 - 2007/1/1

N2 - The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1 degrees C, 4 degrees C, or room temperature and then frozen at -80 degrees C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1 degrees C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4 degrees C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.

AB - The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage in 3 paradigms in which the same postmortem tissue sample (frontal cortex) was frozen a short time after death or stored at 1 degrees C, 4 degrees C, or room temperature and then frozen at -80 degrees C at different intervals. No evidence of protein degradation as revealed with monodimensional gel electrophoresis and Western blotting was observed in samples artificially stored at 1 degrees C and then frozen at different intervals up to 50 hours after death. However, the levels of several proteins were modified in samples stored at 4 degrees C and this effect was more marked in samples stored at room temperature. Two-dimensional gel electrophoresis and mass spectrometry further corroborated these observations and permitted the identification of other proteins vulnerable or resistant to postmortem delay. Finally, gel electrophoresis and Western blotting of sarkosyl-insoluble fractions in Alzheimer disease showed reduced intensity of phospho-tau-specific bands with postmortem delay with the effects being more dramatic when the brain samples were stored at room temperature for long periods. These results emphasize the necessity of reducing the body temperature after death to minimize protein degradation.

KW - Aged

KW - Alzheimer Disease

KW - Blotting, Western

KW - Brain

KW - Brain Chemistry

KW - Electrophoresis, Gel, Two-Dimensional

KW - Europe

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Postmortem Changes

KW - Proteins

KW - Temperature

KW - Time Factors

KW - Tissue Preservation

KW - Tissue Survival

U2 - 10.1097/nen.0b013e31802c3e7d

DO - 10.1097/nen.0b013e31802c3e7d

M3 - SCORING: Journal article

C2 - 17204935

VL - 66

SP - 35

EP - 46

JO - J NEUROPATH EXP NEUR

JF - J NEUROPATH EXP NEUR

SN - 0022-3069

IS - 1

ER -