Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo
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Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo. / Donofrio, Gaetano; Martignani, Eugenio; Poli, Enzo; Lange, Claudia; Martini, Filippo Maria; Cavirani, Sandro; Cabassi, Clotilde Silvia; Taddei, Simone; Flammini, Cesidio Filippo.
In: J VIROL METHODS, Vol. 136, No. 1-2, 01.09.2006, p. 126-36.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo
AU - Donofrio, Gaetano
AU - Martignani, Eugenio
AU - Poli, Enzo
AU - Lange, Claudia
AU - Martini, Filippo Maria
AU - Cavirani, Sandro
AU - Cabassi, Clotilde Silvia
AU - Taddei, Simone
AU - Flammini, Cesidio Filippo
PY - 2006/9/1
Y1 - 2006/9/1
N2 - Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Bovine herpesvirus 4 (BoHV-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. For the in vitro study, a buffalo rat liver cell line was successfully transduced by BoHV-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmacologically selected, but no viral replication took place. For the in vivo study, adult male rats were inoculated intraportally and intraparenchimally with a BoHV-4 expressing enhanced green fluorescent protein and liver sections were analyzed through fluorescent microscopy. Although the liver parenchyma could not be transduced, the endothelial layer of the liver vasculature showed a robust transgene expression without toxicity. Successful BoHV-4 based vector transduction of primary cultures of rat hepatocytes suggests that extrinsic factors, and not hepatocytes per se, are the cause of such lack of transducibility. The present study serves as a starting point for study of the use of BoHV-4 based vectors to target gene delivery to vascular endothelial cells.
AB - Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Bovine herpesvirus 4 (BoHV-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. For the in vitro study, a buffalo rat liver cell line was successfully transduced by BoHV-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmacologically selected, but no viral replication took place. For the in vivo study, adult male rats were inoculated intraportally and intraparenchimally with a BoHV-4 expressing enhanced green fluorescent protein and liver sections were analyzed through fluorescent microscopy. Although the liver parenchyma could not be transduced, the endothelial layer of the liver vasculature showed a robust transgene expression without toxicity. Successful BoHV-4 based vector transduction of primary cultures of rat hepatocytes suggests that extrinsic factors, and not hepatocytes per se, are the cause of such lack of transducibility. The present study serves as a starting point for study of the use of BoHV-4 based vectors to target gene delivery to vascular endothelial cells.
KW - Animals
KW - Cell Line
KW - Endothelial Cells
KW - Gene Transfer Techniques
KW - Genes, Reporter
KW - Genetic Therapy
KW - Genetic Vectors
KW - Green Fluorescent Proteins
KW - Hepatocytes
KW - Herpesvirus 4, Bovine
KW - Liver
KW - Male
KW - Microscopy, Fluorescence
KW - Rats
KW - Rats, Inbred BUF
KW - Staining and Labeling
U2 - 10.1016/j.jviromet.2006.04.008
DO - 10.1016/j.jviromet.2006.04.008
M3 - SCORING: Journal article
C2 - 16712963
VL - 136
SP - 126
EP - 136
JO - J VIROL METHODS
JF - J VIROL METHODS
SN - 0166-0934
IS - 1-2
ER -