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Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo

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Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo. / Donofrio, Gaetano; Martignani, Eugenio; Poli, Enzo; Lange, Claudia; Martini, Filippo Maria; Cavirani, Sandro; Cabassi, Clotilde Silvia; Taddei, Simone; Flammini, Cesidio Filippo.

in: J VIROL METHODS, Jahrgang 136, Nr. 1-2, 01.09.2006, S. 126-36.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Donofrio, G, Martignani, E, Poli, E, Lange, C, Martini, FM, Cavirani, S, Cabassi, CS, Taddei, S & Flammini, CF 2006, 'Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo', J VIROL METHODS, Jg. 136, Nr. 1-2, S. 126-36. https://doi.org/10.1016/j.jviromet.2006.04.008

APA

Donofrio, G., Martignani, E., Poli, E., Lange, C., Martini, F. M., Cavirani, S., Cabassi, C. S., Taddei, S., & Flammini, C. F. (2006). Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo. J VIROL METHODS, 136(1-2), 126-36. https://doi.org/10.1016/j.jviromet.2006.04.008

Vancouver

Donofrio G, Martignani E, Poli E, Lange C, Martini FM, Cavirani S et al. Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo. J VIROL METHODS. 2006 Sep 1;136(1-2):126-36. https://doi.org/10.1016/j.jviromet.2006.04.008

Bibtex

@article{c083e8f2e97347a9b00ccc5768254d58,
title = "Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo",
abstract = "Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Bovine herpesvirus 4 (BoHV-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. For the in vitro study, a buffalo rat liver cell line was successfully transduced by BoHV-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmacologically selected, but no viral replication took place. For the in vivo study, adult male rats were inoculated intraportally and intraparenchimally with a BoHV-4 expressing enhanced green fluorescent protein and liver sections were analyzed through fluorescent microscopy. Although the liver parenchyma could not be transduced, the endothelial layer of the liver vasculature showed a robust transgene expression without toxicity. Successful BoHV-4 based vector transduction of primary cultures of rat hepatocytes suggests that extrinsic factors, and not hepatocytes per se, are the cause of such lack of transducibility. The present study serves as a starting point for study of the use of BoHV-4 based vectors to target gene delivery to vascular endothelial cells.",
keywords = "Animals, Cell Line, Endothelial Cells, Gene Transfer Techniques, Genes, Reporter, Genetic Therapy, Genetic Vectors, Green Fluorescent Proteins, Hepatocytes, Herpesvirus 4, Bovine, Liver, Male, Microscopy, Fluorescence, Rats, Rats, Inbred BUF, Staining and Labeling",
author = "Gaetano Donofrio and Eugenio Martignani and Enzo Poli and Claudia Lange and Martini, {Filippo Maria} and Sandro Cavirani and Cabassi, {Clotilde Silvia} and Simone Taddei and Flammini, {Cesidio Filippo}",
year = "2006",
month = sep,
day = "1",
doi = "10.1016/j.jviromet.2006.04.008",
language = "English",
volume = "136",
pages = "126--36",
journal = "J VIROL METHODS",
issn = "0166-0934",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo

AU - Donofrio, Gaetano

AU - Martignani, Eugenio

AU - Poli, Enzo

AU - Lange, Claudia

AU - Martini, Filippo Maria

AU - Cavirani, Sandro

AU - Cabassi, Clotilde Silvia

AU - Taddei, Simone

AU - Flammini, Cesidio Filippo

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Bovine herpesvirus 4 (BoHV-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. For the in vitro study, a buffalo rat liver cell line was successfully transduced by BoHV-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmacologically selected, but no viral replication took place. For the in vivo study, adult male rats were inoculated intraportally and intraparenchimally with a BoHV-4 expressing enhanced green fluorescent protein and liver sections were analyzed through fluorescent microscopy. Although the liver parenchyma could not be transduced, the endothelial layer of the liver vasculature showed a robust transgene expression without toxicity. Successful BoHV-4 based vector transduction of primary cultures of rat hepatocytes suggests that extrinsic factors, and not hepatocytes per se, are the cause of such lack of transducibility. The present study serves as a starting point for study of the use of BoHV-4 based vectors to target gene delivery to vascular endothelial cells.

AB - Gene transfer into hepatocytes is highly desirable for the long-term goal of replacing deficient proteins and correcting metabolic disorders. Bovine herpesvirus 4 (BoHV-4) based vector capability to transduce rat liver cells in vitro and in vivo was assessed. For the in vitro study, a buffalo rat liver cell line was successfully transduced by BoHV-4 and although did not show toxicity, the immediate early two viral gene was transcribed and cells harboring the intact viral genome could be pharmacologically selected, but no viral replication took place. For the in vivo study, adult male rats were inoculated intraportally and intraparenchimally with a BoHV-4 expressing enhanced green fluorescent protein and liver sections were analyzed through fluorescent microscopy. Although the liver parenchyma could not be transduced, the endothelial layer of the liver vasculature showed a robust transgene expression without toxicity. Successful BoHV-4 based vector transduction of primary cultures of rat hepatocytes suggests that extrinsic factors, and not hepatocytes per se, are the cause of such lack of transducibility. The present study serves as a starting point for study of the use of BoHV-4 based vectors to target gene delivery to vascular endothelial cells.

KW - Animals

KW - Cell Line

KW - Endothelial Cells

KW - Gene Transfer Techniques

KW - Genes, Reporter

KW - Genetic Therapy

KW - Genetic Vectors

KW - Green Fluorescent Proteins

KW - Hepatocytes

KW - Herpesvirus 4, Bovine

KW - Liver

KW - Male

KW - Microscopy, Fluorescence

KW - Rats

KW - Rats, Inbred BUF

KW - Staining and Labeling

U2 - 10.1016/j.jviromet.2006.04.008

DO - 10.1016/j.jviromet.2006.04.008

M3 - SCORING: Journal article

C2 - 16712963

VL - 136

SP - 126

EP - 136

JO - J VIROL METHODS

JF - J VIROL METHODS

SN - 0166-0934

IS - 1-2

ER -