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Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells

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Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. / Ancelin, Katia; Lange, Ulrike C; Hajkova, Petra; Schneider, Robert; Bannister, Andrew J; Kouzarides, Tony; Surani, M Azim.

In: NAT CELL BIOL, Vol. 8, No. 6, 01.06.2006, p. 623-30.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ancelin, K, Lange, UC, Hajkova, P, Schneider, R, Bannister, AJ, Kouzarides, T & Surani, MA 2006, 'Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells', NAT CELL BIOL, vol. 8, no. 6, pp. 623-30. https://doi.org/10.1038/ncb1413

APA

Ancelin, K., Lange, U. C., Hajkova, P., Schneider, R., Bannister, A. J., Kouzarides, T., & Surani, M. A. (2006). Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. NAT CELL BIOL, 8(6), 623-30. https://doi.org/10.1038/ncb1413

Vancouver

Ancelin K, Lange UC, Hajkova P, Schneider R, Bannister AJ, Kouzarides T et al. Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. NAT CELL BIOL. 2006 Jun 1;8(6):623-30. https://doi.org/10.1038/ncb1413

Bibtex

@article{93a1f38e70ce43688547f5e8b1783dd8,
title = "Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells",
abstract = "Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.",
keywords = "Active Transport, Cell Nucleus, Adenosine Triphosphatases, Age Factors, Animals, Arginine, Embryo, Mammalian, Gene Expression Regulation, Germ Cells, Histones, Methylation, Mice, Protein Binding, Protein Methyltransferases, Repressor Proteins, Transcription Factors, Transcription, Genetic",
author = "Katia Ancelin and Lange, {Ulrike C} and Petra Hajkova and Robert Schneider and Bannister, {Andrew J} and Tony Kouzarides and Surani, {M Azim}",
year = "2006",
month = jun,
day = "1",
doi = "10.1038/ncb1413",
language = "English",
volume = "8",
pages = "623--30",
journal = "NAT CELL BIOL",
issn = "1465-7392",
publisher = "NATURE PUBLISHING GROUP",
number = "6",

}

RIS

TY - JOUR

T1 - Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells

AU - Ancelin, Katia

AU - Lange, Ulrike C

AU - Hajkova, Petra

AU - Schneider, Robert

AU - Bannister, Andrew J

AU - Kouzarides, Tony

AU - Surani, M Azim

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.

AB - Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.

KW - Active Transport, Cell Nucleus

KW - Adenosine Triphosphatases

KW - Age Factors

KW - Animals

KW - Arginine

KW - Embryo, Mammalian

KW - Gene Expression Regulation

KW - Germ Cells

KW - Histones

KW - Methylation

KW - Mice

KW - Protein Binding

KW - Protein Methyltransferases

KW - Repressor Proteins

KW - Transcription Factors

KW - Transcription, Genetic

U2 - 10.1038/ncb1413

DO - 10.1038/ncb1413

M3 - SCORING: Journal article

C2 - 16699504

VL - 8

SP - 623

EP - 630

JO - NAT CELL BIOL

JF - NAT CELL BIOL

SN - 1465-7392

IS - 6

ER -