Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells
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Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. / Ancelin, Katia; Lange, Ulrike C; Hajkova, Petra; Schneider, Robert; Bannister, Andrew J; Kouzarides, Tony; Surani, M Azim.
in: NAT CELL BIOL, Jahrgang 8, Nr. 6, 01.06.2006, S. 623-30.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells
AU - Ancelin, Katia
AU - Lange, Ulrike C
AU - Hajkova, Petra
AU - Schneider, Robert
AU - Bannister, Andrew J
AU - Kouzarides, Tony
AU - Surani, M Azim
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.
AB - Blimp1, a transcriptional repressor, has a crucial role in the specification of primordial germ cells (PGCs) in mice at embryonic day 7.5 (E7.5). This SET-PR domain protein can form complexes with various chromatin modifiers in a context-dependent manner. Here, we show that Blimp1 has a novel interaction with Prmt5, an arginine-specific histone methyltransferase, which mediates symmetrical dimethylation of arginine 3 on histone H2A and/or H4 tails (H2A/H4R3me2s). Prmt5 has been shown to associate with Tudor, a component of germ plasm in Drosophila melanogaster. Blimp1-Prmt5 colocalization results in high levels of H2A/H4 R3 methylation in PGCs at E8.5. However, at E11.5, Blimp1-Prmt5 translocates from the nucleus to the cytoplasm, resulting in the loss of H2A/H4 R3 methylation at the time of extensive epigenetic reprogramming of germ cells. Subsequently, Dhx38, a putative target of the Blimp1-Prmt5 complex, is upregulated. Interestingly, expression of Dhx38 is also seen in pluripotent embryonic germ cells that are derived from PGCs when Blimp1 expression is lost. Our study demonstrates that Blimp1 is involved in a novel transcriptional regulatory complex in the mouse germ-cell lineage.
KW - Active Transport, Cell Nucleus
KW - Adenosine Triphosphatases
KW - Age Factors
KW - Animals
KW - Arginine
KW - Embryo, Mammalian
KW - Gene Expression Regulation
KW - Germ Cells
KW - Histones
KW - Methylation
KW - Mice
KW - Protein Binding
KW - Protein Methyltransferases
KW - Repressor Proteins
KW - Transcription Factors
KW - Transcription, Genetic
U2 - 10.1038/ncb1413
DO - 10.1038/ncb1413
M3 - SCORING: Journal article
C2 - 16699504
VL - 8
SP - 623
EP - 630
JO - NAT CELL BIOL
JF - NAT CELL BIOL
SN - 1465-7392
IS - 6
ER -