Best practices for time-resolved serial synchrotron crystallography
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Best practices for time-resolved serial synchrotron crystallography. / Schulz, Eike C; Yorke, Briony A; Pearson, Arwen R; Mehrabi, Pedram.
In: ACTA CRYSTALLOGR D, Vol. 78, No. 1, 01.01.2022, p. 14-29.Research output: SCORING: Contribution to journal › SCORING: Review article › Research
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TY - JOUR
T1 - Best practices for time-resolved serial synchrotron crystallography
AU - Schulz, Eike C
AU - Yorke, Briony A
AU - Pearson, Arwen R
AU - Mehrabi, Pedram
N1 - open access.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just that: discrete experiments, not just `data collections'. As such, careful planning and consideration of potential pitfalls is required to enable a successful experiment. Here, some of the key factors that should be considered during the planning and execution of a time-resolved structural study are outlined, with a particular focus on synchrotron-based experiments.
AB - With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just that: discrete experiments, not just `data collections'. As such, careful planning and consideration of potential pitfalls is required to enable a successful experiment. Here, some of the key factors that should be considered during the planning and execution of a time-resolved structural study are outlined, with a particular focus on synchrotron-based experiments.
KW - Animals
KW - Crystallography, X-Ray/instrumentation
KW - Data Analysis
KW - Enzymes/chemistry
KW - Humans
KW - Synchrotrons
U2 - 10.1107/S2059798321011621
DO - 10.1107/S2059798321011621
M3 - SCORING: Review article
C2 - 34981758
VL - 78
SP - 14
EP - 29
JO - ACTA CRYSTALLOGR D
JF - ACTA CRYSTALLOGR D
SN - 2059-7983
IS - 1
ER -