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BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.

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BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. / Dieni, Sandra; Matsumoto, Tomoya; Dekkers, Martijn; Rauskolb, Stefanie; Ionescu, Mihai S; Deogracias, Ruben; Gundelfinger, Eckart D; Kojima, Masami; Nestel, Sigrun; Frotscher, Michael; Barde, Yves-Alain.

In: J CELL BIOL, Vol. 196, No. 6, 6, 2012, p. 775-788.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dieni, S, Matsumoto, T, Dekkers, M, Rauskolb, S, Ionescu, MS, Deogracias, R, Gundelfinger, ED, Kojima, M, Nestel, S, Frotscher, M & Barde, Y-A 2012, 'BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.', J CELL BIOL, vol. 196, no. 6, 6, pp. 775-788. <http://www.ncbi.nlm.nih.gov/pubmed/22412021?dopt=Citation>

APA

Dieni, S., Matsumoto, T., Dekkers, M., Rauskolb, S., Ionescu, M. S., Deogracias, R., Gundelfinger, E. D., Kojima, M., Nestel, S., Frotscher, M., & Barde, Y-A. (2012). BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. J CELL BIOL, 196(6), 775-788. [6]. http://www.ncbi.nlm.nih.gov/pubmed/22412021?dopt=Citation

Vancouver

Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R et al. BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. J CELL BIOL. 2012;196(6):775-788. 6.

Bibtex

@article{cd47bb8885574e7ea4aa96ba47bbaa9f,
title = "BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.",
abstract = "Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ~10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.",
author = "Sandra Dieni and Tomoya Matsumoto and Martijn Dekkers and Stefanie Rauskolb and Ionescu, {Mihai S} and Ruben Deogracias and Gundelfinger, {Eckart D} and Masami Kojima and Sigrun Nestel and Michael Frotscher and Yves-Alain Barde",
year = "2012",
language = "English",
volume = "196",
pages = "775--788",
journal = "J CELL BIOL",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "6",

}

RIS

TY - JOUR

T1 - BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.

AU - Dieni, Sandra

AU - Matsumoto, Tomoya

AU - Dekkers, Martijn

AU - Rauskolb, Stefanie

AU - Ionescu, Mihai S

AU - Deogracias, Ruben

AU - Gundelfinger, Eckart D

AU - Kojima, Masami

AU - Nestel, Sigrun

AU - Frotscher, Michael

AU - Barde, Yves-Alain

PY - 2012

Y1 - 2012

N2 - Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ~10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.

AB - Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ~10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.

M3 - SCORING: Journal article

VL - 196

SP - 775

EP - 788

JO - J CELL BIOL

JF - J CELL BIOL

SN - 0021-9525

IS - 6

M1 - 6

ER -