BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
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BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. / Dieni, Sandra; Matsumoto, Tomoya; Dekkers, Martijn; Rauskolb, Stefanie; Ionescu, Mihai S; Deogracias, Ruben; Gundelfinger, Eckart D; Kojima, Masami; Nestel, Sigrun; Frotscher, Michael; Barde, Yves-Alain.
in: J CELL BIOL, Jahrgang 196, Nr. 6, 6, 2012, S. 775-788.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
AU - Dieni, Sandra
AU - Matsumoto, Tomoya
AU - Dekkers, Martijn
AU - Rauskolb, Stefanie
AU - Ionescu, Mihai S
AU - Deogracias, Ruben
AU - Gundelfinger, Eckart D
AU - Kojima, Masami
AU - Nestel, Sigrun
AU - Frotscher, Michael
AU - Barde, Yves-Alain
PY - 2012
Y1 - 2012
N2 - Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ~10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.
AB - Although brain-derived neurotrophic factor (BDNF) regulates numerous and complex biological processes including memory retention, its extremely low levels in the mature central nervous system have greatly complicated attempts to reliably localize it. Using rigorous specificity controls, we found that antibodies reacting either with BDNF or its pro-peptide both stained large dense core vesicles in excitatory presynaptic terminals of the adult mouse hippocampus. Both moieties were ~10-fold more abundant than pro-BDNF. The lack of postsynaptic localization was confirmed in Bassoon mutants, a seizure-prone mouse line exhibiting markedly elevated levels of BDNF. These findings challenge previous conclusions based on work with cultured neurons, which suggested activity-dependent dendritic synthesis and release of BDNF. They instead provide an ultrastructural basis for an anterograde mode of action of BDNF, contrasting with the long-established retrograde model derived from experiments with nerve growth factor in the peripheral nervous system.
M3 - SCORING: Journal article
VL - 196
SP - 775
EP - 788
JO - J CELL BIOL
JF - J CELL BIOL
SN - 0021-9525
IS - 6
M1 - 6
ER -