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Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

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Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta. / Tediashvili, Grigol; Wang, Dong; Reichenspurner, Hermann; Deuse, Tobias; Schrepfer, Sonja.

In: JOVE-J VIS EXP, No. 132, e56477, 07.02.2018.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Tediashvili, G, Wang, D, Reichenspurner, H, Deuse, T & Schrepfer, S 2018, 'Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta', JOVE-J VIS EXP, no. 132, e56477. https://doi.org/10.3791/56477

APA

Tediashvili, G., Wang, D., Reichenspurner, H., Deuse, T., & Schrepfer, S. (2018). Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta. JOVE-J VIS EXP, (132), [e56477]. https://doi.org/10.3791/56477

Vancouver

Tediashvili G, Wang D, Reichenspurner H, Deuse T, Schrepfer S. Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta. JOVE-J VIS EXP. 2018 Feb 7;(132). e56477. https://doi.org/10.3791/56477

Bibtex

@article{744c9195d07a4990b12f5cb877cddc5a,
title = "Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta",
abstract = "The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.",
keywords = "Animals, Aorta, Abdominal/pathology, Hyperplasia/pathology, Mice, Models, Animal, Tunica Intima/pathology",
author = "Grigol Tediashvili and Dong Wang and Hermann Reichenspurner and Tobias Deuse and Sonja Schrepfer",
year = "2018",
month = feb,
day = "7",
doi = "10.3791/56477",
language = "English",
journal = "JOVE-J VIS EXP",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "132",

}

RIS

TY - JOUR

T1 - Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

AU - Tediashvili, Grigol

AU - Wang, Dong

AU - Reichenspurner, Hermann

AU - Deuse, Tobias

AU - Schrepfer, Sonja

PY - 2018/2/7

Y1 - 2018/2/7

N2 - The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.

AB - The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.

KW - Animals

KW - Aorta, Abdominal/pathology

KW - Hyperplasia/pathology

KW - Mice

KW - Models, Animal

KW - Tunica Intima/pathology

U2 - 10.3791/56477

DO - 10.3791/56477

M3 - SCORING: Journal article

C2 - 29443065

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 132

M1 - e56477

ER -