Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta
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Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta. / Tediashvili, Grigol; Wang, Dong; Reichenspurner, Hermann; Deuse, Tobias; Schrepfer, Sonja.
in: JOVE-J VIS EXP, Nr. 132, e56477, 07.02.2018.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta
AU - Tediashvili, Grigol
AU - Wang, Dong
AU - Reichenspurner, Hermann
AU - Deuse, Tobias
AU - Schrepfer, Sonja
PY - 2018/2/7
Y1 - 2018/2/7
N2 - The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.
AB - The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.
KW - Animals
KW - Aorta, Abdominal/pathology
KW - Hyperplasia/pathology
KW - Mice
KW - Models, Animal
KW - Tunica Intima/pathology
U2 - 10.3791/56477
DO - 10.3791/56477
M3 - SCORING: Journal article
C2 - 29443065
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
IS - 132
M1 - e56477
ER -