Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage
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Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage. / Gutensohn, K; Vossen, D; Strate, A; Kersten, J F; Hofbauer, M; Krieger, T.
In: INT J LAB HEMATOL, Vol. 35, No. 2, 2, 2013, p. 150-162.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage
AU - Gutensohn, K
AU - Vossen, D
AU - Strate, A
AU - Kersten, J F
AU - Hofbauer, M
AU - Krieger, T
N1 - © 2012 Blackwell Publishing Ltd.
PY - 2013
Y1 - 2013
N2 - INTRODUCTION: Anti-cardiolipin and β2-glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi-automated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2 -glycoprotein I antibodies were tested.METHODS: A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.RESULTS: For both anti-cardiolipin and anti-β2 -glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.CONCLUSION: Our study revealed a moderate to poor accordance between five different test systems for anti-cardiolipin and anti-β2 -glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti-phospholipid antibodies should be achieved.
AB - INTRODUCTION: Anti-cardiolipin and β2-glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi-automated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2 -glycoprotein I antibodies were tested.METHODS: A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.RESULTS: For both anti-cardiolipin and anti-β2 -glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.CONCLUSION: Our study revealed a moderate to poor accordance between five different test systems for anti-cardiolipin and anti-β2 -glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti-phospholipid antibodies should be achieved.
KW - Abortion, Spontaneous
KW - Adult
KW - Antibodies, Antiphospholipid
KW - Automation
KW - Clinical Chemistry Tests
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Pregnancy
KW - beta 2-Glycoprotein I
U2 - 10.1111/ijlh.12011
DO - 10.1111/ijlh.12011
M3 - SCORING: Journal article
C2 - 23033892
VL - 35
SP - 150
EP - 162
JO - INT J LAB HEMATOL
JF - INT J LAB HEMATOL
SN - 1751-5521
IS - 2
M1 - 2
ER -