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Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage

Standard

Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage. / Gutensohn, K; Vossen, D; Strate, A; Kersten, J F; Hofbauer, M; Krieger, T.

in: INT J LAB HEMATOL, Jahrgang 35, Nr. 2, 2, 2013, S. 150-162.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gutensohn, K, Vossen, D, Strate, A, Kersten, JF, Hofbauer, M & Krieger, T 2013, 'Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage', INT J LAB HEMATOL, Jg. 35, Nr. 2, 2, S. 150-162. https://doi.org/10.1111/ijlh.12011

APA

Gutensohn, K., Vossen, D., Strate, A., Kersten, J. F., Hofbauer, M., & Krieger, T. (2013). Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage. INT J LAB HEMATOL, 35(2), 150-162. [2]. https://doi.org/10.1111/ijlh.12011

Vancouver

Gutensohn K, Vossen D, Strate A, Kersten JF, Hofbauer M, Krieger T. Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage. INT J LAB HEMATOL. 2013;35(2):150-162. 2. https://doi.org/10.1111/ijlh.12011

Bibtex

@article{1c945faa0d60419cb237c9a800fe1b83,
title = "Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage",
abstract = "INTRODUCTION: Anti-cardiolipin and β2-glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi-automated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2 -glycoprotein I antibodies were tested.METHODS: A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.RESULTS: For both anti-cardiolipin and anti-β2 -glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.CONCLUSION: Our study revealed a moderate to poor accordance between five different test systems for anti-cardiolipin and anti-β2 -glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti-phospholipid antibodies should be achieved.",
keywords = "Abortion, Spontaneous, Adult, Antibodies, Antiphospholipid, Automation, Clinical Chemistry Tests, Enzyme-Linked Immunosorbent Assay, Female, Humans, Pregnancy, beta 2-Glycoprotein I",
author = "K Gutensohn and D Vossen and A Strate and Kersten, {J F} and M Hofbauer and T Krieger",
note = "{\textcopyright} 2012 Blackwell Publishing Ltd.",
year = "2013",
doi = "10.1111/ijlh.12011",
language = "English",
volume = "35",
pages = "150--162",
journal = "INT J LAB HEMATOL",
issn = "1751-5521",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Automated, semi-automated, and manual analyses of anti-cardiolipin and anti-β2-glycoprotein I antibodies in women with a history of miscarriage

AU - Gutensohn, K

AU - Vossen, D

AU - Strate, A

AU - Kersten, J F

AU - Hofbauer, M

AU - Krieger, T

N1 - © 2012 Blackwell Publishing Ltd.

PY - 2013

Y1 - 2013

N2 - INTRODUCTION: Anti-cardiolipin and β2-glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi-automated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2 -glycoprotein I antibodies were tested.METHODS: A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.RESULTS: For both anti-cardiolipin and anti-β2 -glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.CONCLUSION: Our study revealed a moderate to poor accordance between five different test systems for anti-cardiolipin and anti-β2 -glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti-phospholipid antibodies should be achieved.

AB - INTRODUCTION: Anti-cardiolipin and β2-glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi-automated, and manual immunoassays detecting IgG/IgM anti-cardiolipin and anti-β2 -glycoprotein I antibodies were tested.METHODS: A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.RESULTS: For both anti-cardiolipin and anti-β2 -glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.CONCLUSION: Our study revealed a moderate to poor accordance between five different test systems for anti-cardiolipin and anti-β2 -glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti-phospholipid antibodies should be achieved.

KW - Abortion, Spontaneous

KW - Adult

KW - Antibodies, Antiphospholipid

KW - Automation

KW - Clinical Chemistry Tests

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Pregnancy

KW - beta 2-Glycoprotein I

U2 - 10.1111/ijlh.12011

DO - 10.1111/ijlh.12011

M3 - SCORING: Journal article

C2 - 23033892

VL - 35

SP - 150

EP - 162

JO - INT J LAB HEMATOL

JF - INT J LAB HEMATOL

SN - 1751-5521

IS - 2

M1 - 2

ER -