Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)

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Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL). / Worthmann, Anna; John, Clara; Heeren, Joerg.

In: BIO-PROTOCOL, Vol. 8, No. 13, 05.07.2018, p. e2916.

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@article{530649b66078455898226a56bf837fb3,
title = "Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)",
abstract = "The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.",
author = "Anna Worthmann and Clara John and Joerg Heeren",
note = "Copyright {\textcopyright} 2018 The Authors; exclusive licensee Bio-protocol LLC.",
year = "2018",
month = jul,
day = "5",
doi = "10.21769/BioProtoc.2916",
language = "English",
volume = "8",
pages = "e2916",
journal = "BIO-PROTOCOL",
issn = "2331-8325",
publisher = "Sunnyvale, CA",
number = "13",

}

RIS

TY - JOUR

T1 - Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)

AU - Worthmann, Anna

AU - John, Clara

AU - Heeren, Joerg

N1 - Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

PY - 2018/7/5

Y1 - 2018/7/5

N2 - The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.

AB - The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.

U2 - 10.21769/BioProtoc.2916

DO - 10.21769/BioProtoc.2916

M3 - SCORING: Journal article

C2 - 34395745

VL - 8

SP - e2916

JO - BIO-PROTOCOL

JF - BIO-PROTOCOL

SN - 2331-8325

IS - 13

ER -