Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)
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Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL). / Worthmann, Anna; John, Clara; Heeren, Joerg.
In: BIO-PROTOCOL, Vol. 8, No. 13, 05.07.2018, p. e2916.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)
AU - Worthmann, Anna
AU - John, Clara
AU - Heeren, Joerg
N1 - Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.
PY - 2018/7/5
Y1 - 2018/7/5
N2 - The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.
AB - The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.
U2 - 10.21769/BioProtoc.2916
DO - 10.21769/BioProtoc.2916
M3 - SCORING: Journal article
C2 - 34395745
VL - 8
SP - e2916
JO - BIO-PROTOCOL
JF - BIO-PROTOCOL
SN - 2331-8325
IS - 13
ER -