Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)

Abstract

The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice housed at different ambient temperatures.

Bibliographical data

Original languageEnglish
ISSN2331-8325
DOIs
Publication statusPublished - 05.07.2018

Comment Deanary

Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

PubMed 34395745