Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice

Amol Bhandare; Joseph van de Wiel; Reno Roberts; Ingke Braren; Robert Huckstepp; Nicholas Dale

doi:10.7554/eLife.70671

Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice

Standard

Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice. / Bhandare, Amol; van de Wiel, Joseph; Roberts, Reno; Braren, Ingke; Huckstepp, Robert; Dale, Nicholas.

In: ELIFE, Vol. 11, e70671, 27.10.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bhandare, A, van de Wiel, J, Roberts, R, Braren, I, Huckstepp, R & Dale, N 2022, 'Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice', ELIFE, vol. 11, e70671. https://doi.org/10.7554/eLife.70671

APA

Bhandare, A., van de Wiel, J., Roberts, R., Braren, I., Huckstepp, R., & Dale, N. (2022). Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice. ELIFE, 11, [e70671]. https://doi.org/10.7554/eLife.70671

Vancouver

Bhandare A, van de Wiel J, Roberts R, Braren I, Huckstepp R, Dale N. Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice. ELIFE. 2022 Oct 27;11. e70671. https://doi.org/10.7554/eLife.70671

Bibtex

@article{b010428e83ae4ba8884d0c9adf2f6bbe,

title = "Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice",

abstract = "Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here, we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.",

keywords = "Mice, Animals, Hypercapnia/metabolism, Carbon Dioxide/metabolism, Medulla Oblongata/physiology, Brain Stem/physiology, Respiration",

author = "Amol Bhandare and {van de Wiel}, Joseph and Reno Roberts and Ingke Braren and Robert Huckstepp and Nicholas Dale",

note = "{\textcopyright} 2022, Bhandare et al.",

year = "2022",

month = oct,

day = "27",

doi = "10.7554/eLife.70671",

language = "English",

volume = "11",

journal = "ELIFE",

issn = "2050-084X",

publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice

AU - Bhandare, Amol

AU - van de Wiel, Joseph

AU - Roberts, Reno

AU - Braren, Ingke

AU - Huckstepp, Robert

AU - Dale, Nicholas

PY - 2022/10/27

Y1 - 2022/10/27

N2 - Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here, we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.

AB - Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here, we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.

KW - Mice

KW - Animals

KW - Hypercapnia/metabolism

KW - Carbon Dioxide/metabolism

KW - Medulla Oblongata/physiology

KW - Brain Stem/physiology

KW - Respiration

U2 - 10.7554/eLife.70671

DO - 10.7554/eLife.70671

M3 - SCORING: Journal article

C2 - 36300918

VL - 11

JO - ELIFE

JF - ELIFE

SN - 2050-084X

M1 - e70671

ER -