Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
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Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice. / Bhandare, Amol; van de Wiel, Joseph; Roberts, Reno; Braren, Ingke; Huckstepp, Robert; Dale, Nicholas.
in: ELIFE, Jahrgang 11, e70671, 27.10.2022.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice
AU - Bhandare, Amol
AU - van de Wiel, Joseph
AU - Roberts, Reno
AU - Braren, Ingke
AU - Huckstepp, Robert
AU - Dale, Nicholas
N1 - © 2022, Bhandare et al.
PY - 2022/10/27
Y1 - 2022/10/27
N2 - Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here, we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.
AB - Regulation of systemic PCO2 is a life-preserving homeostatic mechanism. In the medulla oblongata, the retrotrapezoid nucleus (RTN) and rostral medullary Raphe are proposed as CO2 chemosensory nuclei mediating adaptive respiratory changes. Hypercapnia also induces active expiration, an adaptive change thought to be controlled by the lateral parafacial region (pFL). Here, we use GCaMP6 expression and head-mounted mini-microscopes to image Ca2+ activity in these nuclei in awake adult mice during hypercapnia. Activity in the pFL supports its role as a homogenous neuronal population that drives active expiration. Our data show that chemosensory responses in the RTN and Raphe differ in their temporal characteristics and sensitivity to CO2, raising the possibility these nuclei act in a coordinated way to generate adaptive ventilatory responses to hypercapnia. Our analysis revises the understanding of chemosensory control in awake adult mouse and paves the way to understanding how breathing is coordinated with complex non-ventilatory behaviours.
KW - Mice
KW - Animals
KW - Hypercapnia/metabolism
KW - Carbon Dioxide/metabolism
KW - Medulla Oblongata/physiology
KW - Brain Stem/physiology
KW - Respiration
U2 - 10.7554/eLife.70671
DO - 10.7554/eLife.70671
M3 - SCORING: Journal article
C2 - 36300918
VL - 11
JO - ELIFE
JF - ELIFE
SN - 2050-084X
M1 - e70671
ER -