Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome
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Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome. / Butler, Lynn Marie; Hallström, Björn Mikael; Fagerberg, Linn; Pontén, Fredrik; Uhlén, Mathias; Renné, Thomas; Odeberg, Jacob.
In: CELL SYST, Vol. 3, No. 3, 28.09.2016, p. 287-301.e3.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome
AU - Butler, Lynn Marie
AU - Hallström, Björn Mikael
AU - Fagerberg, Linn
AU - Pontén, Fredrik
AU - Uhlén, Mathias
AU - Renné, Thomas
AU - Odeberg, Jacob
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/9/28
Y1 - 2016/9/28
N2 - Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.
AB - Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.
U2 - 10.1016/j.cels.2016.08.001
DO - 10.1016/j.cels.2016.08.001
M3 - SCORING: Journal article
C2 - 27641958
VL - 3
SP - 287-301.e3
JO - CELL SYST
JF - CELL SYST
SN - 2405-4712
IS - 3
ER -