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Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome

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Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome. / Butler, Lynn Marie; Hallström, Björn Mikael; Fagerberg, Linn; Pontén, Fredrik; Uhlén, Mathias; Renné, Thomas; Odeberg, Jacob.

in: CELL SYST, Jahrgang 3, Nr. 3, 28.09.2016, S. 287-301.e3.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Butler, LM, Hallström, BM, Fagerberg, L, Pontén, F, Uhlén, M, Renné, T & Odeberg, J 2016, 'Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome', CELL SYST, Jg. 3, Nr. 3, S. 287-301.e3. https://doi.org/10.1016/j.cels.2016.08.001

APA

Butler, L. M., Hallström, B. M., Fagerberg, L., Pontén, F., Uhlén, M., Renné, T., & Odeberg, J. (2016). Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome. CELL SYST, 3(3), 287-301.e3. https://doi.org/10.1016/j.cels.2016.08.001

Vancouver

Butler LM, Hallström BM, Fagerberg L, Pontén F, Uhlén M, Renné T et al. Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome. CELL SYST. 2016 Sep 28;3(3):287-301.e3. https://doi.org/10.1016/j.cels.2016.08.001

Bibtex

@article{2f0e1b08a4d8471097770d8ac4a5888b,
title = "Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome",
abstract = "Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.",
author = "Butler, {Lynn Marie} and Hallstr{\"o}m, {Bj{\"o}rn Mikael} and Linn Fagerberg and Fredrik Pont{\'e}n and Mathias Uhl{\'e}n and Thomas Renn{\'e} and Jacob Odeberg",
note = "Copyright {\textcopyright} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = sep,
day = "28",
doi = "10.1016/j.cels.2016.08.001",
language = "English",
volume = "3",
pages = "287--301.e3",
journal = "CELL SYST",
issn = "2405-4712",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Analysis of Body-wide Unfractionated Tissue Data to Identify a Core Human Endothelial Transcriptome

AU - Butler, Lynn Marie

AU - Hallström, Björn Mikael

AU - Fagerberg, Linn

AU - Pontén, Fredrik

AU - Uhlén, Mathias

AU - Renné, Thomas

AU - Odeberg, Jacob

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/9/28

Y1 - 2016/9/28

N2 - Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.

AB - Endothelial cells line blood vessels and regulate hemostasis, inflammation, and blood pressure. Proteins critical for these specialized functions tend to be predominantly expressed in endothelial cells across vascular beds. Here, we present a systems approach to identify a panel of human endothelial-enriched genes using global, body-wide transcriptomics data from 124 tissue samples from 32 organs. We identified known and unknown endothelial-enriched gene transcripts and used antibody-based profiling to confirm expression across vascular beds. The majority of identified transcripts could be detected in cultured endothelial cells from various vascular beds, and we observed maintenance of relative expression in early passage cells. In summary, we describe a widely applicable method to determine cell-type-specific transcriptome profiles in a whole-organism context, based on differential abundance across tissues. We identify potential vascular drug targets or endothelial biomarkers and highlight candidates for functional studies to increase understanding of the endothelium in health and disease.

U2 - 10.1016/j.cels.2016.08.001

DO - 10.1016/j.cels.2016.08.001

M3 - SCORING: Journal article

C2 - 27641958

VL - 3

SP - 287-301.e3

JO - CELL SYST

JF - CELL SYST

SN - 2405-4712

IS - 3

ER -