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An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy

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An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy. / Hoxha, Elion; Beck, Laurence H; Wiech, Thorsten; Tomas, Nicola M; Probst, Christian; Mindorf, Swantje; Meyer-Schwesinger, Catherine; Zahner, Gunther; Stahl, Phillip R; Schöpper, Ruth; Panzer, Ulf; Harendza, Sigrid; Helmchen, Udo; Salant, David J; Stahl, Rolf A K.

In: J AM SOC NEPHROL, Vol. 28, No. 2, 02.2017, p. 520-531.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hoxha, E, Beck, LH, Wiech, T, Tomas, NM, Probst, C, Mindorf, S, Meyer-Schwesinger, C, Zahner, G, Stahl, PR, Schöpper, R, Panzer, U, Harendza, S, Helmchen, U, Salant, DJ & Stahl, RAK 2017, 'An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy', J AM SOC NEPHROL, vol. 28, no. 2, pp. 520-531. https://doi.org/10.1681/ASN.2016010050

APA

Hoxha, E., Beck, L. H., Wiech, T., Tomas, N. M., Probst, C., Mindorf, S., Meyer-Schwesinger, C., Zahner, G., Stahl, P. R., Schöpper, R., Panzer, U., Harendza, S., Helmchen, U., Salant, D. J., & Stahl, R. A. K. (2017). An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy. J AM SOC NEPHROL, 28(2), 520-531. https://doi.org/10.1681/ASN.2016010050

Vancouver

Hoxha E, Beck LH, Wiech T, Tomas NM, Probst C, Mindorf S et al. An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy. J AM SOC NEPHROL. 2017 Feb;28(2):520-531. https://doi.org/10.1681/ASN.2016010050

Bibtex

@article{6bb1da8857ca42be83b0b57d2fc76bfa,
title = "An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy",
abstract = "Thrombospondin type 1 domain-containing 7A (THSD7A) is a target antigen identified in adult membranous nephropathy (MN) along with the major antigen phospholipase A2 receptor 1 (PLA2R1). The prevalence of THSD7A-Ab-positive patients is unknown, and it is unclear whether the clinical presentation differs between patients positive for PLA2R1-Ab or THSD7A-Ab. We screened serum samples of 1276 patients with MN from three different cohorts for the presence of THSD7A-Ab by Western blot analysis and a newly developed indirect immunofluorescence test (IFT). Compared with Western blot analysis, the IFT had a 92% sensitivity and a 100% specificity. The prevalence of THSD7A-associated MN in a prospective cohort of 345 patients with MN was 2.6%, and most were women. In this cohort, the percentage of patients with THSD7A-associated MN and malignant disease significantly exceeded that of patients with PLA2R1-associated MN and malignant disease. In all cohorts, we identified 40 patients with THSD7A-associated MN, eight of whom developed a malignancy within a median time of 3 months from diagnosis of MN. In one patient with THSD7A-associated MN and metastases of an endometrial carcinoma, immunohistochemistry showed THSD7A expression on the metastatic cells and within follicular dendritic cells of the metastasis-infiltrated lymph node. We conclude that the IFT allows sensitive and specific measurement of circulating THSD7A-Ab in patients with MN. Patients with THSD7A-associated MN differ in their clinical characteristics from patients with PLA2R1-associated MN, and more intensive screening for the presence of malignancies may be warranted in those with THSD7A-associated MN.",
author = "Elion Hoxha and Beck, {Laurence H} and Thorsten Wiech and Tomas, {Nicola M} and Christian Probst and Swantje Mindorf and Catherine Meyer-Schwesinger and Gunther Zahner and Stahl, {Phillip R} and Ruth Sch{\"o}pper and Ulf Panzer and Sigrid Harendza and Udo Helmchen and Salant, {David J} and Stahl, {Rolf A K}",
note = "Copyright {\textcopyright} 2016 by the American Society of Nephrology.",
year = "2017",
month = feb,
doi = "10.1681/ASN.2016010050",
language = "English",
volume = "28",
pages = "520--531",
journal = "J AM SOC NEPHROL",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "2",

}

RIS

TY - JOUR

T1 - An Indirect Immunofluorescence Method Facilitates Detection of Thrombospondin Type 1 Domain-Containing 7A-Specific Antibodies in Membranous Nephropathy

AU - Hoxha, Elion

AU - Beck, Laurence H

AU - Wiech, Thorsten

AU - Tomas, Nicola M

AU - Probst, Christian

AU - Mindorf, Swantje

AU - Meyer-Schwesinger, Catherine

AU - Zahner, Gunther

AU - Stahl, Phillip R

AU - Schöpper, Ruth

AU - Panzer, Ulf

AU - Harendza, Sigrid

AU - Helmchen, Udo

AU - Salant, David J

AU - Stahl, Rolf A K

N1 - Copyright © 2016 by the American Society of Nephrology.

PY - 2017/2

Y1 - 2017/2

N2 - Thrombospondin type 1 domain-containing 7A (THSD7A) is a target antigen identified in adult membranous nephropathy (MN) along with the major antigen phospholipase A2 receptor 1 (PLA2R1). The prevalence of THSD7A-Ab-positive patients is unknown, and it is unclear whether the clinical presentation differs between patients positive for PLA2R1-Ab or THSD7A-Ab. We screened serum samples of 1276 patients with MN from three different cohorts for the presence of THSD7A-Ab by Western blot analysis and a newly developed indirect immunofluorescence test (IFT). Compared with Western blot analysis, the IFT had a 92% sensitivity and a 100% specificity. The prevalence of THSD7A-associated MN in a prospective cohort of 345 patients with MN was 2.6%, and most were women. In this cohort, the percentage of patients with THSD7A-associated MN and malignant disease significantly exceeded that of patients with PLA2R1-associated MN and malignant disease. In all cohorts, we identified 40 patients with THSD7A-associated MN, eight of whom developed a malignancy within a median time of 3 months from diagnosis of MN. In one patient with THSD7A-associated MN and metastases of an endometrial carcinoma, immunohistochemistry showed THSD7A expression on the metastatic cells and within follicular dendritic cells of the metastasis-infiltrated lymph node. We conclude that the IFT allows sensitive and specific measurement of circulating THSD7A-Ab in patients with MN. Patients with THSD7A-associated MN differ in their clinical characteristics from patients with PLA2R1-associated MN, and more intensive screening for the presence of malignancies may be warranted in those with THSD7A-associated MN.

AB - Thrombospondin type 1 domain-containing 7A (THSD7A) is a target antigen identified in adult membranous nephropathy (MN) along with the major antigen phospholipase A2 receptor 1 (PLA2R1). The prevalence of THSD7A-Ab-positive patients is unknown, and it is unclear whether the clinical presentation differs between patients positive for PLA2R1-Ab or THSD7A-Ab. We screened serum samples of 1276 patients with MN from three different cohorts for the presence of THSD7A-Ab by Western blot analysis and a newly developed indirect immunofluorescence test (IFT). Compared with Western blot analysis, the IFT had a 92% sensitivity and a 100% specificity. The prevalence of THSD7A-associated MN in a prospective cohort of 345 patients with MN was 2.6%, and most were women. In this cohort, the percentage of patients with THSD7A-associated MN and malignant disease significantly exceeded that of patients with PLA2R1-associated MN and malignant disease. In all cohorts, we identified 40 patients with THSD7A-associated MN, eight of whom developed a malignancy within a median time of 3 months from diagnosis of MN. In one patient with THSD7A-associated MN and metastases of an endometrial carcinoma, immunohistochemistry showed THSD7A expression on the metastatic cells and within follicular dendritic cells of the metastasis-infiltrated lymph node. We conclude that the IFT allows sensitive and specific measurement of circulating THSD7A-Ab in patients with MN. Patients with THSD7A-associated MN differ in their clinical characteristics from patients with PLA2R1-associated MN, and more intensive screening for the presence of malignancies may be warranted in those with THSD7A-associated MN.

U2 - 10.1681/ASN.2016010050

DO - 10.1681/ASN.2016010050

M3 - SCORING: Journal article

C2 - 27436855

VL - 28

SP - 520

EP - 531

JO - J AM SOC NEPHROL

JF - J AM SOC NEPHROL

SN - 1046-6673

IS - 2

ER -