Research ExplorerPublicationsAlternatively spliced transcripts of the thymus-specific pro...

Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus

Standard

Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus. / Luther, C; Wienhold, W; Oehlmann, R; Heinemann, M K; Melms, A; Tolosa, E.

In: GENES IMMUN, Vol. 6, No. 1, 01.02.2005, p. 1-7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Luther, C, Wienhold, W, Oehlmann, R, Heinemann, MK, Melms, A & Tolosa, E 2005, 'Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus', GENES IMMUN, vol. 6, no. 1, pp. 1-7. https://doi.org/10.1038/sj.gene.6364142

APA

Luther, C., Wienhold, W., Oehlmann, R., Heinemann, M. K., Melms, A., & Tolosa, E. (2005). Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus. GENES IMMUN, 6(1), 1-7. https://doi.org/10.1038/sj.gene.6364142

Vancouver

Luther C, Wienhold W, Oehlmann R, Heinemann MK, Melms A, Tolosa E. Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus. GENES IMMUN. 2005 Feb 1;6(1):1-7. https://doi.org/10.1038/sj.gene.6364142

Bibtex

@article{316c1c4e6d194dcc94ee61752f30c735,
title = "Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus",
abstract = "The putative serine protease PRSS16 is abundantly expressed in the thymic cortex and the gene is encoded within the HLA I complex. Although its function is not yet defined, the very restricted expression points to a role in T-cell development in the thymus. In this study, we show that the PRSS16 mRNA is alternatively spliced to generate at least five transcripts. Apart from the full-length sequence, we found two other isoforms with all putative active site residues of the serine protease, suggesting that those variants may also be functional. Semi-quantitative analysis of the splice variants in different tissue samples revealed a strong correlation between the specific formation of alternatively spliced PRSS16 transcripts and the age and thymus pathology status of the donor. Newborn thymi express mostly the PRSS16-4 and -5 isoforms and lack the PRSS16-1 transcript, which appears around 2 years of age and stays until adulthood. Incidentally, thymi from myasthenia gravis (MG) patients with thymoma showed a marked decrease in the expression of the full-length PRSS16-1 and increased expression of the smaller isoforms. The data suggest a potential role of the PRSS16 isoforms in the postnatal morphogenesis of the thymus and in the thymus pathology related to MG.",
keywords = "Adult, Alternative Splicing, Base Sequence, Female, Gene Expression Regulation, Enzymologic, Humans, Isoenzymes, Male, Middle Aged, Molecular Sequence Data, Morphogenesis, Myasthenia Gravis, Serine Endopeptidases, Thymus Gland",
author = "C Luther and W Wienhold and R Oehlmann and Heinemann, {M K} and A Melms and E Tolosa",
year = "2005",
month = feb,
day = "1",
doi = "10.1038/sj.gene.6364142",
language = "English",
volume = "6",
pages = "1--7",
journal = "GENES IMMUN",
issn = "1466-4879",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus

AU - Luther, C

AU - Wienhold, W

AU - Oehlmann, R

AU - Heinemann, M K

AU - Melms, A

AU - Tolosa, E

PY - 2005/2/1

Y1 - 2005/2/1

N2 - The putative serine protease PRSS16 is abundantly expressed in the thymic cortex and the gene is encoded within the HLA I complex. Although its function is not yet defined, the very restricted expression points to a role in T-cell development in the thymus. In this study, we show that the PRSS16 mRNA is alternatively spliced to generate at least five transcripts. Apart from the full-length sequence, we found two other isoforms with all putative active site residues of the serine protease, suggesting that those variants may also be functional. Semi-quantitative analysis of the splice variants in different tissue samples revealed a strong correlation between the specific formation of alternatively spliced PRSS16 transcripts and the age and thymus pathology status of the donor. Newborn thymi express mostly the PRSS16-4 and -5 isoforms and lack the PRSS16-1 transcript, which appears around 2 years of age and stays until adulthood. Incidentally, thymi from myasthenia gravis (MG) patients with thymoma showed a marked decrease in the expression of the full-length PRSS16-1 and increased expression of the smaller isoforms. The data suggest a potential role of the PRSS16 isoforms in the postnatal morphogenesis of the thymus and in the thymus pathology related to MG.

AB - The putative serine protease PRSS16 is abundantly expressed in the thymic cortex and the gene is encoded within the HLA I complex. Although its function is not yet defined, the very restricted expression points to a role in T-cell development in the thymus. In this study, we show that the PRSS16 mRNA is alternatively spliced to generate at least five transcripts. Apart from the full-length sequence, we found two other isoforms with all putative active site residues of the serine protease, suggesting that those variants may also be functional. Semi-quantitative analysis of the splice variants in different tissue samples revealed a strong correlation between the specific formation of alternatively spliced PRSS16 transcripts and the age and thymus pathology status of the donor. Newborn thymi express mostly the PRSS16-4 and -5 isoforms and lack the PRSS16-1 transcript, which appears around 2 years of age and stays until adulthood. Incidentally, thymi from myasthenia gravis (MG) patients with thymoma showed a marked decrease in the expression of the full-length PRSS16-1 and increased expression of the smaller isoforms. The data suggest a potential role of the PRSS16 isoforms in the postnatal morphogenesis of the thymus and in the thymus pathology related to MG.

KW - Adult

KW - Alternative Splicing

KW - Base Sequence

KW - Female

KW - Gene Expression Regulation, Enzymologic

KW - Humans

KW - Isoenzymes

KW - Male

KW - Middle Aged

KW - Molecular Sequence Data

KW - Morphogenesis

KW - Myasthenia Gravis

KW - Serine Endopeptidases

KW - Thymus Gland

U2 - 10.1038/sj.gene.6364142

DO - 10.1038/sj.gene.6364142

M3 - SCORING: Journal article

C2 - 15592422

VL - 6

SP - 1

EP - 7

JO - GENES IMMUN

JF - GENES IMMUN

SN - 1466-4879

IS - 1

ER -