Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus
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Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus. / Luther, C; Wienhold, W; Oehlmann, R; Heinemann, M K; Melms, A; Tolosa, E.
in: GENES IMMUN, Jahrgang 6, Nr. 1, 01.02.2005, S. 1-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Alternatively spliced transcripts of the thymus-specific protease PRSS16 are differentially expressed in human thymus
AU - Luther, C
AU - Wienhold, W
AU - Oehlmann, R
AU - Heinemann, M K
AU - Melms, A
AU - Tolosa, E
PY - 2005/2/1
Y1 - 2005/2/1
N2 - The putative serine protease PRSS16 is abundantly expressed in the thymic cortex and the gene is encoded within the HLA I complex. Although its function is not yet defined, the very restricted expression points to a role in T-cell development in the thymus. In this study, we show that the PRSS16 mRNA is alternatively spliced to generate at least five transcripts. Apart from the full-length sequence, we found two other isoforms with all putative active site residues of the serine protease, suggesting that those variants may also be functional. Semi-quantitative analysis of the splice variants in different tissue samples revealed a strong correlation between the specific formation of alternatively spliced PRSS16 transcripts and the age and thymus pathology status of the donor. Newborn thymi express mostly the PRSS16-4 and -5 isoforms and lack the PRSS16-1 transcript, which appears around 2 years of age and stays until adulthood. Incidentally, thymi from myasthenia gravis (MG) patients with thymoma showed a marked decrease in the expression of the full-length PRSS16-1 and increased expression of the smaller isoforms. The data suggest a potential role of the PRSS16 isoforms in the postnatal morphogenesis of the thymus and in the thymus pathology related to MG.
AB - The putative serine protease PRSS16 is abundantly expressed in the thymic cortex and the gene is encoded within the HLA I complex. Although its function is not yet defined, the very restricted expression points to a role in T-cell development in the thymus. In this study, we show that the PRSS16 mRNA is alternatively spliced to generate at least five transcripts. Apart from the full-length sequence, we found two other isoforms with all putative active site residues of the serine protease, suggesting that those variants may also be functional. Semi-quantitative analysis of the splice variants in different tissue samples revealed a strong correlation between the specific formation of alternatively spliced PRSS16 transcripts and the age and thymus pathology status of the donor. Newborn thymi express mostly the PRSS16-4 and -5 isoforms and lack the PRSS16-1 transcript, which appears around 2 years of age and stays until adulthood. Incidentally, thymi from myasthenia gravis (MG) patients with thymoma showed a marked decrease in the expression of the full-length PRSS16-1 and increased expression of the smaller isoforms. The data suggest a potential role of the PRSS16 isoforms in the postnatal morphogenesis of the thymus and in the thymus pathology related to MG.
KW - Adult
KW - Alternative Splicing
KW - Base Sequence
KW - Female
KW - Gene Expression Regulation, Enzymologic
KW - Humans
KW - Isoenzymes
KW - Male
KW - Middle Aged
KW - Molecular Sequence Data
KW - Morphogenesis
KW - Myasthenia Gravis
KW - Serine Endopeptidases
KW - Thymus Gland
U2 - 10.1038/sj.gene.6364142
DO - 10.1038/sj.gene.6364142
M3 - SCORING: Journal article
C2 - 15592422
VL - 6
SP - 1
EP - 7
JO - GENES IMMUN
JF - GENES IMMUN
SN - 1466-4879
IS - 1
ER -