Alterations in Rev-ERBα/BMAL1 ratio and glycated hemoglobin in rotating shift workers: the EuRhythDia study
Standard
Alterations in Rev-ERBα/BMAL1 ratio and glycated hemoglobin in rotating shift workers: the EuRhythDia study. / Rizza, Stefano; Luzi, Alessio; Mavilio, Maria; Ballanti, Marta; Massimi, Arianna; Porzio, Ottavia; Magrini, Andrea; Hannemann, Juliane; Menghini, Rossella; Lehrke, Michael; Staels, Bart; Grant, Peter J; Böger, Rainer H; Marx, Nikolaus; Federici, Massimo.
In: ACTA DIABETOL, Vol. 58, No. 8, 08.2021, p. 1111-1117.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Alterations in Rev-ERBα/BMAL1 ratio and glycated hemoglobin in rotating shift workers: the EuRhythDia study
AU - Rizza, Stefano
AU - Luzi, Alessio
AU - Mavilio, Maria
AU - Ballanti, Marta
AU - Massimi, Arianna
AU - Porzio, Ottavia
AU - Magrini, Andrea
AU - Hannemann, Juliane
AU - Menghini, Rossella
AU - Lehrke, Michael
AU - Staels, Bart
AU - Grant, Peter J
AU - Böger, Rainer H
AU - Marx, Nikolaus
AU - Federici, Massimo
N1 - © 2021. The Author(s).
PY - 2021/8
Y1 - 2021/8
N2 - OBJECTIVE: To detect premature gluco-metabolic defects among night shift workers with disturbances in circadian rhythms.DESIGN AND METHODS: We performed a hypothesis-generating, cross-sectional analysis of anthropometric, metabolic, lipid, and inflammation parameters, comparing active (a-NSW, n = 111) and former (f-NSW, n = 98) rotating night shift workers with diurnal workers (controls, n = 69). All participants were hospital nurses. We also evaluated the Pittsburgh Sleep Quality Index (PSQI) and assessed expression of transcription factors REV-ERBα and BMAL1 in peripheral blood mononuclear cells (PBMCs), as indicators of the molecular clock.RESULTS: Both a-NSW and f-NSW participants had significantly higher glycated hemoglobin (HbA1c) and white blood cell counts (WBC) (p < 0.001 for both), PSQI global score (p = 0.001) and diastolic blood pressure levels (p = 0.024) compared with controls. Expression of REV-ERBα/BMAL1 RNA in PBMC was significantly higher in a-NSW (p = 0.05) than in f-NSW or control participants. Multivariate regression analysis showed that working status and PSQI were independent determinants of higher HbA1c levels (p < 0.001).CONCLUSIONS: We demonstrated that young, healthy night shift workers show subclinical abnormalities in HbA1c and changes in peripheral clock gene expression.
AB - OBJECTIVE: To detect premature gluco-metabolic defects among night shift workers with disturbances in circadian rhythms.DESIGN AND METHODS: We performed a hypothesis-generating, cross-sectional analysis of anthropometric, metabolic, lipid, and inflammation parameters, comparing active (a-NSW, n = 111) and former (f-NSW, n = 98) rotating night shift workers with diurnal workers (controls, n = 69). All participants were hospital nurses. We also evaluated the Pittsburgh Sleep Quality Index (PSQI) and assessed expression of transcription factors REV-ERBα and BMAL1 in peripheral blood mononuclear cells (PBMCs), as indicators of the molecular clock.RESULTS: Both a-NSW and f-NSW participants had significantly higher glycated hemoglobin (HbA1c) and white blood cell counts (WBC) (p < 0.001 for both), PSQI global score (p = 0.001) and diastolic blood pressure levels (p = 0.024) compared with controls. Expression of REV-ERBα/BMAL1 RNA in PBMC was significantly higher in a-NSW (p = 0.05) than in f-NSW or control participants. Multivariate regression analysis showed that working status and PSQI were independent determinants of higher HbA1c levels (p < 0.001).CONCLUSIONS: We demonstrated that young, healthy night shift workers show subclinical abnormalities in HbA1c and changes in peripheral clock gene expression.
KW - ARNTL Transcription Factors/blood
KW - Adult
KW - Blood Pressure
KW - Circadian Rhythm/genetics
KW - Cross-Sectional Studies
KW - Female
KW - Gene Expression
KW - Glycated Hemoglobin A/analysis
KW - Hospitals
KW - Humans
KW - Inflammation
KW - Leukocyte Count
KW - Leukocytes, Mononuclear/chemistry
KW - Male
KW - Middle Aged
KW - Nuclear Receptor Subfamily 1, Group D, Member 1/blood
KW - Nurses
KW - RNA/blood
KW - Shift Work Schedule
KW - Sleep/physiology
U2 - 10.1007/s00592-021-01676-z
DO - 10.1007/s00592-021-01676-z
M3 - SCORING: Journal article
C2 - 33788000
VL - 58
SP - 1111
EP - 1117
JO - ACTA DIABETOL
JF - ACTA DIABETOL
SN - 0940-5429
IS - 8
ER -