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Age-related changes in intracellular cytokine expression in healthy children

Standard

Age-related changes in intracellular cytokine expression in healthy children. / Wiegering, Verena; Eyrich, Matthias; Wunder, Christian; Günther, Helga; Schlegel, Paul G; Winkler, Beate.

In: EUR CYTOKINE NETW, Vol. 20, No. 2, 06.2009, p. 75-80.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wiegering, V, Eyrich, M, Wunder, C, Günther, H, Schlegel, PG & Winkler, B 2009, 'Age-related changes in intracellular cytokine expression in healthy children', EUR CYTOKINE NETW, vol. 20, no. 2, pp. 75-80. https://doi.org/10.1684/ecn.2009.0149

APA

Wiegering, V., Eyrich, M., Wunder, C., Günther, H., Schlegel, P. G., & Winkler, B. (2009). Age-related changes in intracellular cytokine expression in healthy children. EUR CYTOKINE NETW, 20(2), 75-80. https://doi.org/10.1684/ecn.2009.0149

Vancouver

Wiegering V, Eyrich M, Wunder C, Günther H, Schlegel PG, Winkler B. Age-related changes in intracellular cytokine expression in healthy children. EUR CYTOKINE NETW. 2009 Jun;20(2):75-80. https://doi.org/10.1684/ecn.2009.0149

Bibtex

@article{b4f8ee7d716d409a8fc8148d145e4dab,
title = "Age-related changes in intracellular cytokine expression in healthy children",
abstract = "Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.",
keywords = "Adolescent, Aging/metabolism, Child, Child, Preschool, Cytokines/biosynthesis, Female, Fetal Blood/cytology, Flow Cytometry, Humans, Immune System/growth & development, Immunologic Memory, Immunophenotyping, Infant, Infant, Newborn, Male, Reference Values, Sex Characteristics, T-Lymphocyte Subsets/immunology",
author = "Verena Wiegering and Matthias Eyrich and Christian Wunder and Helga G{\"u}nther and Schlegel, {Paul G} and Beate Winkler",
year = "2009",
month = jun,
doi = "10.1684/ecn.2009.0149",
language = "English",
volume = "20",
pages = "75--80",
journal = "EUR CYTOKINE NETW",
issn = "1952-4005",
publisher = "John Libbey Eurotext",
number = "2",

}

RIS

TY - JOUR

T1 - Age-related changes in intracellular cytokine expression in healthy children

AU - Wiegering, Verena

AU - Eyrich, Matthias

AU - Wunder, Christian

AU - Günther, Helga

AU - Schlegel, Paul G

AU - Winkler, Beate

PY - 2009/6

Y1 - 2009/6

N2 - Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.

AB - Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.

KW - Adolescent

KW - Aging/metabolism

KW - Child

KW - Child, Preschool

KW - Cytokines/biosynthesis

KW - Female

KW - Fetal Blood/cytology

KW - Flow Cytometry

KW - Humans

KW - Immune System/growth & development

KW - Immunologic Memory

KW - Immunophenotyping

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Reference Values

KW - Sex Characteristics

KW - T-Lymphocyte Subsets/immunology

U2 - 10.1684/ecn.2009.0149

DO - 10.1684/ecn.2009.0149

M3 - SCORING: Journal article

C2 - 19541593

VL - 20

SP - 75

EP - 80

JO - EUR CYTOKINE NETW

JF - EUR CYTOKINE NETW

SN - 1952-4005

IS - 2

ER -