Age-related changes in intracellular cytokine expression in healthy children
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Age-related changes in intracellular cytokine expression in healthy children. / Wiegering, Verena; Eyrich, Matthias; Wunder, Christian; Günther, Helga; Schlegel, Paul G; Winkler, Beate.
in: EUR CYTOKINE NETW, Jahrgang 20, Nr. 2, 06.2009, S. 75-80.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Age-related changes in intracellular cytokine expression in healthy children
AU - Wiegering, Verena
AU - Eyrich, Matthias
AU - Wunder, Christian
AU - Günther, Helga
AU - Schlegel, Paul G
AU - Winkler, Beate
PY - 2009/6
Y1 - 2009/6
N2 - Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.
AB - Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.
KW - Adolescent
KW - Aging/metabolism
KW - Child
KW - Child, Preschool
KW - Cytokines/biosynthesis
KW - Female
KW - Fetal Blood/cytology
KW - Flow Cytometry
KW - Humans
KW - Immune System/growth & development
KW - Immunologic Memory
KW - Immunophenotyping
KW - Infant
KW - Infant, Newborn
KW - Male
KW - Reference Values
KW - Sex Characteristics
KW - T-Lymphocyte Subsets/immunology
U2 - 10.1684/ecn.2009.0149
DO - 10.1684/ecn.2009.0149
M3 - SCORING: Journal article
C2 - 19541593
VL - 20
SP - 75
EP - 80
JO - EUR CYTOKINE NETW
JF - EUR CYTOKINE NETW
SN - 1952-4005
IS - 2
ER -