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Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry

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Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry. / Solano, Maria Emilia; Thiele, Kristin; Stelzer, Ina Annelies; Mittrücker, Hans-Willi; Arck, Petra Clara.

In: Chimerism, Vol. 0, No. 0, 30.10.2014, p. 1-4.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Solano, ME, Thiele, K, Stelzer, IA, Mittrücker, H-W & Arck, PC 2014, 'Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry', Chimerism, vol. 0, no. 0, pp. 1-4. https://doi.org/10.4161/19381956.2014.959827

APA

Solano, M. E., Thiele, K., Stelzer, I. A., Mittrücker, H-W., & Arck, P. C. (2014). Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry. Chimerism, 0(0), 1-4. https://doi.org/10.4161/19381956.2014.959827

Vancouver

Solano ME, Thiele K, Stelzer IA, Mittrücker H-W, Arck PC. Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry. Chimerism. 2014 Oct 30;0(0):1-4. https://doi.org/10.4161/19381956.2014.959827

Bibtex

@article{6dfdb96e73364e588959c87793aef6f1,
title = "Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry",
abstract = "Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.",
author = "Solano, {Maria Emilia} and Kristin Thiele and Stelzer, {Ina Annelies} and Hans-Willi Mittr{\"u}cker and Arck, {Petra Clara}",
note = "Article Addendum",
year = "2014",
month = oct,
day = "30",
doi = "10.4161/19381956.2014.959827",
language = "English",
volume = "0",
pages = "1--4",
journal = "Chimerism",
issn = "1938-1964",
publisher = "LANDES BIOSCIENCE",
number = "0",

}

RIS

TY - JOUR

T1 - Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry

AU - Solano, Maria Emilia

AU - Thiele, Kristin

AU - Stelzer, Ina Annelies

AU - Mittrücker, Hans-Willi

AU - Arck, Petra Clara

N1 - Article Addendum

PY - 2014/10/30

Y1 - 2014/10/30

N2 - Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.

AB - Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.

U2 - 10.4161/19381956.2014.959827

DO - 10.4161/19381956.2014.959827

M3 - SCORING: Journal article

C2 - 25483743

VL - 0

SP - 1

EP - 4

JO - Chimerism

JF - Chimerism

SN - 1938-1964

IS - 0

ER -