Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry
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Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry. / Solano, Maria Emilia; Thiele, Kristin; Stelzer, Ina Annelies; Mittrücker, Hans-Willi; Arck, Petra Clara.
in: Chimerism, Jahrgang 0, Nr. 0, 30.10.2014, S. 1-4.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry
AU - Solano, Maria Emilia
AU - Thiele, Kristin
AU - Stelzer, Ina Annelies
AU - Mittrücker, Hans-Willi
AU - Arck, Petra Clara
N1 - Article Addendum
PY - 2014/10/30
Y1 - 2014/10/30
N2 - Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.
AB - Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.
U2 - 10.4161/19381956.2014.959827
DO - 10.4161/19381956.2014.959827
M3 - SCORING: Journal article
C2 - 25483743
VL - 0
SP - 1
EP - 4
JO - Chimerism
JF - Chimerism
SN - 1938-1964
IS - 0
ER -