Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity
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Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity. / Schuettauf, Frank; Vorwerk, Christian; Naskar, Rita; Orlin, Anton; Quinto, Kristine; Zurakowski, David; Dejneka, Nadine S; Klein, Ronald L; Meyer, Edward M; Bennett, Jean.
In: CURR EYE RES, Vol. 29, No. 6, 12.2004, p. 379-86.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity
AU - Schuettauf, Frank
AU - Vorwerk, Christian
AU - Naskar, Rita
AU - Orlin, Anton
AU - Quinto, Kristine
AU - Zurakowski, David
AU - Dejneka, Nadine S
AU - Klein, Ronald L
AU - Meyer, Edward M
AU - Bennett, Jean
PY - 2004/12
Y1 - 2004/12
N2 - PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity.METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated.RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush.CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.
AB - PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity.METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated.RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush.CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.
KW - Animals
KW - Apoptosis
KW - Brain-Derived Neurotrophic Factor/genetics
KW - Cell Survival/physiology
KW - Cytoprotection
KW - Dependovirus/genetics
KW - Excitatory Amino Acid Agonists/toxicity
KW - Fibroblast Growth Factor 2/genetics
KW - Gene Expression Regulation/physiology
KW - Genetic Therapy/methods
KW - Genetic Vectors
KW - Green Fluorescent Proteins/genetics
KW - N-Methylaspartate/toxicity
KW - Nerve Crush
KW - Neuroprotective Agents
KW - Optic Nerve/pathology
KW - Rats
KW - Rats, Sprague-Dawley
KW - Retinal Diseases/metabolism
KW - Retinal Ganglion Cells/drug effects
KW - Transfection
U2 - 10.1080/02713680490517872
DO - 10.1080/02713680490517872
M3 - SCORING: Journal article
C2 - 15764082
VL - 29
SP - 379
EP - 386
JO - CURR EYE RES
JF - CURR EYE RES
SN - 0271-3683
IS - 6
ER -