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Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity

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Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity. / Schuettauf, Frank; Vorwerk, Christian; Naskar, Rita; Orlin, Anton; Quinto, Kristine; Zurakowski, David; Dejneka, Nadine S; Klein, Ronald L; Meyer, Edward M; Bennett, Jean.

in: CURR EYE RES, Jahrgang 29, Nr. 6, 12.2004, S. 379-86.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schuettauf, F, Vorwerk, C, Naskar, R, Orlin, A, Quinto, K, Zurakowski, D, Dejneka, NS, Klein, RL, Meyer, EM & Bennett, J 2004, 'Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity', CURR EYE RES, Jg. 29, Nr. 6, S. 379-86. https://doi.org/10.1080/02713680490517872

APA

Schuettauf, F., Vorwerk, C., Naskar, R., Orlin, A., Quinto, K., Zurakowski, D., Dejneka, N. S., Klein, R. L., Meyer, E. M., & Bennett, J. (2004). Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity. CURR EYE RES, 29(6), 379-86. https://doi.org/10.1080/02713680490517872

Vancouver

Schuettauf F, Vorwerk C, Naskar R, Orlin A, Quinto K, Zurakowski D et al. Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity. CURR EYE RES. 2004 Dez;29(6):379-86. https://doi.org/10.1080/02713680490517872

Bibtex

@article{8ef5741cbf2449a0a304bfd0c99f3267,
title = "Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity",
abstract = "PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity.METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated.RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush.CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.",
keywords = "Animals, Apoptosis, Brain-Derived Neurotrophic Factor/genetics, Cell Survival/physiology, Cytoprotection, Dependovirus/genetics, Excitatory Amino Acid Agonists/toxicity, Fibroblast Growth Factor 2/genetics, Gene Expression Regulation/physiology, Genetic Therapy/methods, Genetic Vectors, Green Fluorescent Proteins/genetics, N-Methylaspartate/toxicity, Nerve Crush, Neuroprotective Agents, Optic Nerve/pathology, Rats, Rats, Sprague-Dawley, Retinal Diseases/metabolism, Retinal Ganglion Cells/drug effects, Transfection",
author = "Frank Schuettauf and Christian Vorwerk and Rita Naskar and Anton Orlin and Kristine Quinto and David Zurakowski and Dejneka, {Nadine S} and Klein, {Ronald L} and Meyer, {Edward M} and Jean Bennett",
year = "2004",
month = dec,
doi = "10.1080/02713680490517872",
language = "English",
volume = "29",
pages = "379--86",
journal = "CURR EYE RES",
issn = "0271-3683",
publisher = "Taylor & Francis",
number = "6",

}

RIS

TY - JOUR

T1 - Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity

AU - Schuettauf, Frank

AU - Vorwerk, Christian

AU - Naskar, Rita

AU - Orlin, Anton

AU - Quinto, Kristine

AU - Zurakowski, David

AU - Dejneka, Nadine S

AU - Klein, Ronald L

AU - Meyer, Edward M

AU - Bennett, Jean

PY - 2004/12

Y1 - 2004/12

N2 - PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity.METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated.RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush.CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.

AB - PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity.METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated.RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush.CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.

KW - Animals

KW - Apoptosis

KW - Brain-Derived Neurotrophic Factor/genetics

KW - Cell Survival/physiology

KW - Cytoprotection

KW - Dependovirus/genetics

KW - Excitatory Amino Acid Agonists/toxicity

KW - Fibroblast Growth Factor 2/genetics

KW - Gene Expression Regulation/physiology

KW - Genetic Therapy/methods

KW - Genetic Vectors

KW - Green Fluorescent Proteins/genetics

KW - N-Methylaspartate/toxicity

KW - Nerve Crush

KW - Neuroprotective Agents

KW - Optic Nerve/pathology

KW - Rats

KW - Rats, Sprague-Dawley

KW - Retinal Diseases/metabolism

KW - Retinal Ganglion Cells/drug effects

KW - Transfection

U2 - 10.1080/02713680490517872

DO - 10.1080/02713680490517872

M3 - SCORING: Journal article

C2 - 15764082

VL - 29

SP - 379

EP - 386

JO - CURR EYE RES

JF - CURR EYE RES

SN - 0271-3683

IS - 6

ER -