A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae

Bibi Nusreen Imambocus; Andrey Formozov; Fangmin Zhou; Peter Soba

doi:10.1016/j.xpro.2022.101787

A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae

Standard

A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae. / Imambocus, Bibi Nusreen; Formozov, Andrey; Zhou, Fangmin; Soba, Peter.

In: STAR PROTOC, Vol. 3, No. 4, 101787, 16.12.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Imambocus, BN, Formozov, A, Zhou, F & Soba, P 2022, 'A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae', STAR PROTOC, vol. 3, no. 4, 101787. https://doi.org/10.1016/j.xpro.2022.101787

APA

Imambocus, B. N., Formozov, A., Zhou, F., & Soba, P. (2022). A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae. STAR PROTOC, 3(4), [101787]. https://doi.org/10.1016/j.xpro.2022.101787

Vancouver

Imambocus BN, Formozov A, Zhou F, Soba P. A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae. STAR PROTOC. 2022 Dec 16;3(4). 101787. https://doi.org/10.1016/j.xpro.2022.101787

Bibtex

@article{733c118e5ba344aaaa97904eea00607c,

title = "A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae",

abstract = "Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).",

keywords = "Animals, Drosophila melanogaster, Larva, Drosophila, Biological Assay",

author = "Imambocus, {Bibi Nusreen} and Andrey Formozov and Fangmin Zhou and Peter Soba",

note = "{\textcopyright} 2022 The Author(s).",

year = "2022",

month = dec,

day = "16",

doi = "10.1016/j.xpro.2022.101787",

language = "English",

volume = "3",

journal = "STAR PROTOC",

issn = "2666-1667",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae

AU - Imambocus, Bibi Nusreen

AU - Formozov, Andrey

AU - Zhou, Fangmin

AU - Soba, Peter

PY - 2022/12/16

Y1 - 2022/12/16

N2 - Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).

AB - Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).

KW - Animals

KW - Drosophila melanogaster

KW - Larva

KW - Drosophila

KW - Biological Assay

U2 - 10.1016/j.xpro.2022.101787

DO - 10.1016/j.xpro.2022.101787

M3 - SCORING: Journal article

C2 - 36317171

VL - 3

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 4

M1 - 101787

ER -