A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae
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A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae. / Imambocus, Bibi Nusreen; Formozov, Andrey; Zhou, Fangmin; Soba, Peter.
in: STAR PROTOC, Jahrgang 3, Nr. 4, 101787, 16.12.2022.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - A two-choice assay for noxious light avoidance with temporal distribution analysis in Drosophila melanogaster larvae
AU - Imambocus, Bibi Nusreen
AU - Formozov, Andrey
AU - Zhou, Fangmin
AU - Soba, Peter
N1 - © 2022 The Author(s).
PY - 2022/12/16
Y1 - 2022/12/16
N2 - Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).
AB - Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at a specific end point. We provide a detailed protocol to set up light avoidance assays and map the temporal distribution of larvae based on analysis of larval intensities. We describe the assay setup and implementation of scripts for analysis, which can be easily adapted to other two-choice assays and different model organisms. For complete details on the use and execution of this protocol, please refer to Imambocus et al. (2022).
KW - Animals
KW - Drosophila melanogaster
KW - Larva
KW - Drosophila
KW - Biological Assay
U2 - 10.1016/j.xpro.2022.101787
DO - 10.1016/j.xpro.2022.101787
M3 - SCORING: Journal article
C2 - 36317171
VL - 3
JO - STAR PROTOC
JF - STAR PROTOC
SN - 2666-1667
IS - 4
M1 - 101787
ER -