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A strategy for in vitro propagation of rat nephrons

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A strategy for in vitro propagation of rat nephrons. / Steer, Dylan L; Bush, Kevin T; Meyer, Tobias N; Meyer-Schwesinger, Catherine; Nigam, Sanjay K.

In: KIDNEY INT, Vol. 62, No. 6, 01.12.2002, p. 1958-65.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Steer, DL, Bush, KT, Meyer, TN, Meyer-Schwesinger, C & Nigam, SK 2002, 'A strategy for in vitro propagation of rat nephrons', KIDNEY INT, vol. 62, no. 6, pp. 1958-65. https://doi.org/10.1046/j.1523-1755.2002.00694.x

APA

Steer, D. L., Bush, K. T., Meyer, T. N., Meyer-Schwesinger, C., & Nigam, S. K. (2002). A strategy for in vitro propagation of rat nephrons. KIDNEY INT, 62(6), 1958-65. https://doi.org/10.1046/j.1523-1755.2002.00694.x

Vancouver

Steer DL, Bush KT, Meyer TN, Meyer-Schwesinger C, Nigam SK. A strategy for in vitro propagation of rat nephrons. KIDNEY INT. 2002 Dec 1;62(6):1958-65. https://doi.org/10.1046/j.1523-1755.2002.00694.x

Bibtex

@article{ef5a18981ea74726b79b5e8fd2b16710,
title = "A strategy for in vitro propagation of rat nephrons",
abstract = "BACKGROUND: Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing and propagating either whole rat metanephric rudiments or isolated rat UBs. Exploiting the branching program intrinsic to the UB, propagated rat UBs can be recombined with fresh rat mesenchyme to form a large number of rat {"}neokidneys{"} derived from a single progenitor that may be amenable to site-specific modulation of function.METHODS: Whole rat metanephric rudiments or isolated rat UBs were cultured and subdivided through several generations. Both cultured progenitor and subsequent generations of isolated rat UBs were recombined with freshly isolated rat metanephric mesenchyme. The tubules of these rat neokidneys were examined for expression of epithelial markers.RESULTS: Isolated rat UBs and whole rat metanephric rudiments could be propagated through several generations and appeared morphologically identical to their progenitors. Generations of isolated rat UB could be recombined with fresh rat mesenchyme and the resultant neokidney displayed the same morphologic appearance as the whole rat kidney rudiment. The UB-derived and MM-derived portions of the tubules of these rat neokidneys appear contiguous.CONCLUSIONS: The recombination of cultured and propagated rat UB with rat mesenchyme yielded rat neokidneys with tubular structures that appeared morphologically identical to whole rat kidney. In vitro propagation of rat metanephric rudiments and recombination of rat UB and MM suggest the possibility of designing nephrons that possess specific desirable functions that can be propagated in vitro.",
keywords = "Animals, Epithelium, Female, Fetal Tissue Transplantation, Mesoderm, Mice, Nephrons, Organ Culture Techniques, Pregnancy, Rats, Rats, Sprague-Dawley, Transplantation, Heterologous",
author = "Steer, {Dylan L} and Bush, {Kevin T} and Meyer, {Tobias N} and Catherine Meyer-Schwesinger and Nigam, {Sanjay K}",
year = "2002",
month = dec,
day = "1",
doi = "10.1046/j.1523-1755.2002.00694.x",
language = "English",
volume = "62",
pages = "1958--65",
journal = "KIDNEY INT",
issn = "0085-2538",
publisher = "NATURE PUBLISHING GROUP",
number = "6",

}

RIS

TY - JOUR

T1 - A strategy for in vitro propagation of rat nephrons

AU - Steer, Dylan L

AU - Bush, Kevin T

AU - Meyer, Tobias N

AU - Meyer-Schwesinger, Catherine

AU - Nigam, Sanjay K

PY - 2002/12/1

Y1 - 2002/12/1

N2 - BACKGROUND: Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing and propagating either whole rat metanephric rudiments or isolated rat UBs. Exploiting the branching program intrinsic to the UB, propagated rat UBs can be recombined with fresh rat mesenchyme to form a large number of rat "neokidneys" derived from a single progenitor that may be amenable to site-specific modulation of function.METHODS: Whole rat metanephric rudiments or isolated rat UBs were cultured and subdivided through several generations. Both cultured progenitor and subsequent generations of isolated rat UBs were recombined with freshly isolated rat metanephric mesenchyme. The tubules of these rat neokidneys were examined for expression of epithelial markers.RESULTS: Isolated rat UBs and whole rat metanephric rudiments could be propagated through several generations and appeared morphologically identical to their progenitors. Generations of isolated rat UB could be recombined with fresh rat mesenchyme and the resultant neokidney displayed the same morphologic appearance as the whole rat kidney rudiment. The UB-derived and MM-derived portions of the tubules of these rat neokidneys appear contiguous.CONCLUSIONS: The recombination of cultured and propagated rat UB with rat mesenchyme yielded rat neokidneys with tubular structures that appeared morphologically identical to whole rat kidney. In vitro propagation of rat metanephric rudiments and recombination of rat UB and MM suggest the possibility of designing nephrons that possess specific desirable functions that can be propagated in vitro.

AB - BACKGROUND: Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing and propagating either whole rat metanephric rudiments or isolated rat UBs. Exploiting the branching program intrinsic to the UB, propagated rat UBs can be recombined with fresh rat mesenchyme to form a large number of rat "neokidneys" derived from a single progenitor that may be amenable to site-specific modulation of function.METHODS: Whole rat metanephric rudiments or isolated rat UBs were cultured and subdivided through several generations. Both cultured progenitor and subsequent generations of isolated rat UBs were recombined with freshly isolated rat metanephric mesenchyme. The tubules of these rat neokidneys were examined for expression of epithelial markers.RESULTS: Isolated rat UBs and whole rat metanephric rudiments could be propagated through several generations and appeared morphologically identical to their progenitors. Generations of isolated rat UB could be recombined with fresh rat mesenchyme and the resultant neokidney displayed the same morphologic appearance as the whole rat kidney rudiment. The UB-derived and MM-derived portions of the tubules of these rat neokidneys appear contiguous.CONCLUSIONS: The recombination of cultured and propagated rat UB with rat mesenchyme yielded rat neokidneys with tubular structures that appeared morphologically identical to whole rat kidney. In vitro propagation of rat metanephric rudiments and recombination of rat UB and MM suggest the possibility of designing nephrons that possess specific desirable functions that can be propagated in vitro.

KW - Animals

KW - Epithelium

KW - Female

KW - Fetal Tissue Transplantation

KW - Mesoderm

KW - Mice

KW - Nephrons

KW - Organ Culture Techniques

KW - Pregnancy

KW - Rats

KW - Rats, Sprague-Dawley

KW - Transplantation, Heterologous

U2 - 10.1046/j.1523-1755.2002.00694.x

DO - 10.1046/j.1523-1755.2002.00694.x

M3 - SCORING: Journal article

C2 - 12427120

VL - 62

SP - 1958

EP - 1965

JO - KIDNEY INT

JF - KIDNEY INT

SN - 0085-2538

IS - 6

ER -