A strategy for in vitro propagation of rat nephrons
Standard
A strategy for in vitro propagation of rat nephrons. / Steer, Dylan L; Bush, Kevin T; Meyer, Tobias N; Meyer-Schwesinger, Catherine; Nigam, Sanjay K.
in: KIDNEY INT, Jahrgang 62, Nr. 6, 01.12.2002, S. 1958-65.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - A strategy for in vitro propagation of rat nephrons
AU - Steer, Dylan L
AU - Bush, Kevin T
AU - Meyer, Tobias N
AU - Meyer-Schwesinger, Catherine
AU - Nigam, Sanjay K
PY - 2002/12/1
Y1 - 2002/12/1
N2 - BACKGROUND: Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing and propagating either whole rat metanephric rudiments or isolated rat UBs. Exploiting the branching program intrinsic to the UB, propagated rat UBs can be recombined with fresh rat mesenchyme to form a large number of rat "neokidneys" derived from a single progenitor that may be amenable to site-specific modulation of function.METHODS: Whole rat metanephric rudiments or isolated rat UBs were cultured and subdivided through several generations. Both cultured progenitor and subsequent generations of isolated rat UBs were recombined with freshly isolated rat metanephric mesenchyme. The tubules of these rat neokidneys were examined for expression of epithelial markers.RESULTS: Isolated rat UBs and whole rat metanephric rudiments could be propagated through several generations and appeared morphologically identical to their progenitors. Generations of isolated rat UB could be recombined with fresh rat mesenchyme and the resultant neokidney displayed the same morphologic appearance as the whole rat kidney rudiment. The UB-derived and MM-derived portions of the tubules of these rat neokidneys appear contiguous.CONCLUSIONS: The recombination of cultured and propagated rat UB with rat mesenchyme yielded rat neokidneys with tubular structures that appeared morphologically identical to whole rat kidney. In vitro propagation of rat metanephric rudiments and recombination of rat UB and MM suggest the possibility of designing nephrons that possess specific desirable functions that can be propagated in vitro.
AB - BACKGROUND: Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing and propagating either whole rat metanephric rudiments or isolated rat UBs. Exploiting the branching program intrinsic to the UB, propagated rat UBs can be recombined with fresh rat mesenchyme to form a large number of rat "neokidneys" derived from a single progenitor that may be amenable to site-specific modulation of function.METHODS: Whole rat metanephric rudiments or isolated rat UBs were cultured and subdivided through several generations. Both cultured progenitor and subsequent generations of isolated rat UBs were recombined with freshly isolated rat metanephric mesenchyme. The tubules of these rat neokidneys were examined for expression of epithelial markers.RESULTS: Isolated rat UBs and whole rat metanephric rudiments could be propagated through several generations and appeared morphologically identical to their progenitors. Generations of isolated rat UB could be recombined with fresh rat mesenchyme and the resultant neokidney displayed the same morphologic appearance as the whole rat kidney rudiment. The UB-derived and MM-derived portions of the tubules of these rat neokidneys appear contiguous.CONCLUSIONS: The recombination of cultured and propagated rat UB with rat mesenchyme yielded rat neokidneys with tubular structures that appeared morphologically identical to whole rat kidney. In vitro propagation of rat metanephric rudiments and recombination of rat UB and MM suggest the possibility of designing nephrons that possess specific desirable functions that can be propagated in vitro.
KW - Animals
KW - Epithelium
KW - Female
KW - Fetal Tissue Transplantation
KW - Mesoderm
KW - Mice
KW - Nephrons
KW - Organ Culture Techniques
KW - Pregnancy
KW - Rats
KW - Rats, Sprague-Dawley
KW - Transplantation, Heterologous
U2 - 10.1046/j.1523-1755.2002.00694.x
DO - 10.1046/j.1523-1755.2002.00694.x
M3 - SCORING: Journal article
C2 - 12427120
VL - 62
SP - 1958
EP - 1965
JO - KIDNEY INT
JF - KIDNEY INT
SN - 0085-2538
IS - 6
ER -