A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference
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A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference. / Gebrekidan, Medhanie Tesfay; Knipfer, Christian; Stelzle, Florian; Popp, Juergen; Will, Stefan; Braeuer, Andreas Siegfried.
In: J RAMAN SPECTROSC, Vol. 47, No. 2, 2016, p. 198-209.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference
AU - Gebrekidan, Medhanie Tesfay
AU - Knipfer, Christian
AU - Stelzle, Florian
AU - Popp, Juergen
AU - Will, Stefan
AU - Braeuer, Andreas Siegfried
N1 - doi: 10.1002/jrs.4775
PY - 2016
Y1 - 2016
N2 - A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that ? in our experiments ? the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785?nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. ? 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.
AB - A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that ? in our experiments ? the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785?nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. ? 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.
KW - shifted-excitation Raman difference spectroscopy
KW - fluorescence
KW - fluorescence rejection
KW - reconstruction of Raman spectra
U2 - 10.1002/jrs.4775
DO - 10.1002/jrs.4775
M3 - SCORING: Zeitschriftenaufsatz
VL - 47
SP - 198
EP - 209
JO - J RAMAN SPECTROSC
JF - J RAMAN SPECTROSC
SN - 0377-0486
IS - 2
ER -