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A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference

Standard

A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference. / Gebrekidan, Medhanie Tesfay; Knipfer, Christian; Stelzle, Florian; Popp, Juergen; Will, Stefan; Braeuer, Andreas Siegfried.

in: J RAMAN SPECTROSC, Jahrgang 47, Nr. 2, 2016, S. 198-209.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gebrekidan, MT, Knipfer, C, Stelzle, F, Popp, J, Will, S & Braeuer, AS 2016, 'A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference', J RAMAN SPECTROSC, Jg. 47, Nr. 2, S. 198-209. https://doi.org/10.1002/jrs.4775

APA

Gebrekidan, M. T., Knipfer, C., Stelzle, F., Popp, J., Will, S., & Braeuer, A. S. (2016). A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference. J RAMAN SPECTROSC, 47(2), 198-209. https://doi.org/10.1002/jrs.4775

Vancouver

Gebrekidan MT, Knipfer C, Stelzle F, Popp J, Will S, Braeuer AS. A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference. J RAMAN SPECTROSC. 2016;47(2):198-209. https://doi.org/10.1002/jrs.4775

Bibtex

@article{608413b830554d69b5ecd06a05d10f1a,
title = "A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference",
abstract = "A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that ? in our experiments ? the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785?nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. ? 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.",
keywords = "shifted-excitation Raman difference spectroscopy, fluorescence, fluorescence rejection, reconstruction of Raman spectra",
author = "Gebrekidan, {Medhanie Tesfay} and Christian Knipfer and Florian Stelzle and Juergen Popp and Stefan Will and Braeuer, {Andreas Siegfried}",
note = "doi: 10.1002/jrs.4775",
year = "2016",
doi = "10.1002/jrs.4775",
language = "Deutsch",
volume = "47",
pages = "198--209",
journal = "J RAMAN SPECTROSC",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - A shifted-excitation Raman difference spectroscopy (SERDS) evaluation strategy for the efficient isolation of Raman spectra from extreme fluorescence interference

AU - Gebrekidan, Medhanie Tesfay

AU - Knipfer, Christian

AU - Stelzle, Florian

AU - Popp, Juergen

AU - Will, Stefan

AU - Braeuer, Andreas Siegfried

N1 - doi: 10.1002/jrs.4775

PY - 2016

Y1 - 2016

N2 - A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that ? in our experiments ? the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785?nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. ? 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.

AB - A biochemical characterization of pathologies in biological tissue can be provided by Raman spectroscopy. Often, the raw spectrum is severely affected by fluorescence interference. We report and compare various spectra-processing approaches required for the purification of Raman spectra from heavily fluorescence-interfered raw spectra according to the shifted-excitation Raman difference spectroscopy method. These approaches cover the entire spectra-processing chain from the raw spectra to the purified Raman spectra. In detail, we compared (1) area normalization versus z-score normalization, (2) direct reconstruction of the difference spectra versus reconstruction of zero-centered difference spectra and (3) collective baseline correction of the reconstructed spectra versus piecewise baseline correction of the reconstructed spectra and, finally, (4) analyzed the influence of the shift of the excitation wavelength on the quality of the reconstructed spectra. Statistical analysis of the spectra showed that ? in our experiments ? the best results were obtained for the z-score normalization before subtraction of the normalized spectra, followed by zero-centering of the difference spectra before reconstruction and a piecewise baseline correction of the pure Raman spectra. With our equipment, a wavelength shift from 784 to 785?nm provided reconstructed spectra of best quality. The analyzed specimens were different tissue types of pigs, tissue from the oral cavity of humans and a model solution of dye dissolved in ethanol. ? 2015 The Authors. Journal of Raman Spectroscopy published by John Wiley & Sons Ltd.

KW - shifted-excitation Raman difference spectroscopy

KW - fluorescence

KW - fluorescence rejection

KW - reconstruction of Raman spectra

U2 - 10.1002/jrs.4775

DO - 10.1002/jrs.4775

M3 - SCORING: Zeitschriftenaufsatz

VL - 47

SP - 198

EP - 209

JO - J RAMAN SPECTROSC

JF - J RAMAN SPECTROSC

SN - 0377-0486

IS - 2

ER -