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A pilot study of gene expression-based categorization of pancreas transplant biopsies

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A pilot study of gene expression-based categorization of pancreas transplant biopsies. / Luan, Fu L; Trillsch, Fabian; Henger, Anna; Eichinger, Felix; Norman, Silas; Appelman, Henry; Kretzler, Matthias.

In: TRANSPLANTATION, Vol. 87, No. 2, 27.01.2009, p. 222-6.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Luan, FL, Trillsch, F, Henger, A, Eichinger, F, Norman, S, Appelman, H & Kretzler, M 2009, 'A pilot study of gene expression-based categorization of pancreas transplant biopsies', TRANSPLANTATION, vol. 87, no. 2, pp. 222-6. https://doi.org/10.1097/TP.0b013e31818c8fbf

APA

Luan, F. L., Trillsch, F., Henger, A., Eichinger, F., Norman, S., Appelman, H., & Kretzler, M. (2009). A pilot study of gene expression-based categorization of pancreas transplant biopsies. TRANSPLANTATION, 87(2), 222-6. https://doi.org/10.1097/TP.0b013e31818c8fbf

Vancouver

Luan FL, Trillsch F, Henger A, Eichinger F, Norman S, Appelman H et al. A pilot study of gene expression-based categorization of pancreas transplant biopsies. TRANSPLANTATION. 2009 Jan 27;87(2):222-6. https://doi.org/10.1097/TP.0b013e31818c8fbf

Bibtex

@article{616d6c47acce4443938c381869518168,
title = "A pilot study of gene expression-based categorization of pancreas transplant biopsies",
abstract = "Gene expression profiling has emerged as a powerful strategy to define transcriptional mechanism activated in organ transplantation. We performed a pilot feasibility study of mRNA-based pancreas transplant biopsy stratification. The mRNAs expression of 32 genes, observed in renal transplant dysfunction, and 10 pancreas-specific genes were evaluated in 26 pancreas transplant biopsy specimens by quantitative real-time polymerase chain reaction using TaqMan Low Density Array technology. Unsupervised 2D hierarchical clustering segregated the biopsies in two main cluster branches, A and B. Six of seven patients (85.7%) in cluster A and 6 of 19 (31.6%) in cluster B retained functioning pancreas allograft. CD20/MS4A1 mRNA and protein, in addition to CD 3 protein, were detected in four specimens in cluster B. Three of those four pancreas transplants were subsequently lost. Our study demonstrates the potential association of gene expression with clinical outcome of pancreas transplants and justifies further studies in an independent cohort.",
keywords = "Adult, Biopsy, Cluster Analysis, Feasibility Studies, Gene Expression Profiling, Graft Rejection, Graft Survival, Humans, Middle Aged, Oligonucleotide Array Sequence Analysis, Pancreas, Pancreas Transplantation, Pilot Projects, Polymerase Chain Reaction, Predictive Value of Tests, RNA, Messenger, Retrospective Studies, Time Factors, Transplantation, Homologous, Treatment Outcome",
author = "Luan, {Fu L} and Fabian Trillsch and Anna Henger and Felix Eichinger and Silas Norman and Henry Appelman and Matthias Kretzler",
year = "2009",
month = jan,
day = "27",
doi = "10.1097/TP.0b013e31818c8fbf",
language = "English",
volume = "87",
pages = "222--6",
journal = "TRANSPLANTATION",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

RIS

TY - JOUR

T1 - A pilot study of gene expression-based categorization of pancreas transplant biopsies

AU - Luan, Fu L

AU - Trillsch, Fabian

AU - Henger, Anna

AU - Eichinger, Felix

AU - Norman, Silas

AU - Appelman, Henry

AU - Kretzler, Matthias

PY - 2009/1/27

Y1 - 2009/1/27

N2 - Gene expression profiling has emerged as a powerful strategy to define transcriptional mechanism activated in organ transplantation. We performed a pilot feasibility study of mRNA-based pancreas transplant biopsy stratification. The mRNAs expression of 32 genes, observed in renal transplant dysfunction, and 10 pancreas-specific genes were evaluated in 26 pancreas transplant biopsy specimens by quantitative real-time polymerase chain reaction using TaqMan Low Density Array technology. Unsupervised 2D hierarchical clustering segregated the biopsies in two main cluster branches, A and B. Six of seven patients (85.7%) in cluster A and 6 of 19 (31.6%) in cluster B retained functioning pancreas allograft. CD20/MS4A1 mRNA and protein, in addition to CD 3 protein, were detected in four specimens in cluster B. Three of those four pancreas transplants were subsequently lost. Our study demonstrates the potential association of gene expression with clinical outcome of pancreas transplants and justifies further studies in an independent cohort.

AB - Gene expression profiling has emerged as a powerful strategy to define transcriptional mechanism activated in organ transplantation. We performed a pilot feasibility study of mRNA-based pancreas transplant biopsy stratification. The mRNAs expression of 32 genes, observed in renal transplant dysfunction, and 10 pancreas-specific genes were evaluated in 26 pancreas transplant biopsy specimens by quantitative real-time polymerase chain reaction using TaqMan Low Density Array technology. Unsupervised 2D hierarchical clustering segregated the biopsies in two main cluster branches, A and B. Six of seven patients (85.7%) in cluster A and 6 of 19 (31.6%) in cluster B retained functioning pancreas allograft. CD20/MS4A1 mRNA and protein, in addition to CD 3 protein, were detected in four specimens in cluster B. Three of those four pancreas transplants were subsequently lost. Our study demonstrates the potential association of gene expression with clinical outcome of pancreas transplants and justifies further studies in an independent cohort.

KW - Adult

KW - Biopsy

KW - Cluster Analysis

KW - Feasibility Studies

KW - Gene Expression Profiling

KW - Graft Rejection

KW - Graft Survival

KW - Humans

KW - Middle Aged

KW - Oligonucleotide Array Sequence Analysis

KW - Pancreas

KW - Pancreas Transplantation

KW - Pilot Projects

KW - Polymerase Chain Reaction

KW - Predictive Value of Tests

KW - RNA, Messenger

KW - Retrospective Studies

KW - Time Factors

KW - Transplantation, Homologous

KW - Treatment Outcome

U2 - 10.1097/TP.0b013e31818c8fbf

DO - 10.1097/TP.0b013e31818c8fbf

M3 - SCORING: Journal article

C2 - 19155976

VL - 87

SP - 222

EP - 226

JO - TRANSPLANTATION

JF - TRANSPLANTATION

SN - 0041-1337

IS - 2

ER -