A pilot study of gene expression-based categorization of pancreas transplant biopsies
Standard
A pilot study of gene expression-based categorization of pancreas transplant biopsies. / Luan, Fu L; Trillsch, Fabian; Henger, Anna; Eichinger, Felix; Norman, Silas; Appelman, Henry; Kretzler, Matthias.
in: TRANSPLANTATION, Jahrgang 87, Nr. 2, 27.01.2009, S. 222-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - A pilot study of gene expression-based categorization of pancreas transplant biopsies
AU - Luan, Fu L
AU - Trillsch, Fabian
AU - Henger, Anna
AU - Eichinger, Felix
AU - Norman, Silas
AU - Appelman, Henry
AU - Kretzler, Matthias
PY - 2009/1/27
Y1 - 2009/1/27
N2 - Gene expression profiling has emerged as a powerful strategy to define transcriptional mechanism activated in organ transplantation. We performed a pilot feasibility study of mRNA-based pancreas transplant biopsy stratification. The mRNAs expression of 32 genes, observed in renal transplant dysfunction, and 10 pancreas-specific genes were evaluated in 26 pancreas transplant biopsy specimens by quantitative real-time polymerase chain reaction using TaqMan Low Density Array technology. Unsupervised 2D hierarchical clustering segregated the biopsies in two main cluster branches, A and B. Six of seven patients (85.7%) in cluster A and 6 of 19 (31.6%) in cluster B retained functioning pancreas allograft. CD20/MS4A1 mRNA and protein, in addition to CD 3 protein, were detected in four specimens in cluster B. Three of those four pancreas transplants were subsequently lost. Our study demonstrates the potential association of gene expression with clinical outcome of pancreas transplants and justifies further studies in an independent cohort.
AB - Gene expression profiling has emerged as a powerful strategy to define transcriptional mechanism activated in organ transplantation. We performed a pilot feasibility study of mRNA-based pancreas transplant biopsy stratification. The mRNAs expression of 32 genes, observed in renal transplant dysfunction, and 10 pancreas-specific genes were evaluated in 26 pancreas transplant biopsy specimens by quantitative real-time polymerase chain reaction using TaqMan Low Density Array technology. Unsupervised 2D hierarchical clustering segregated the biopsies in two main cluster branches, A and B. Six of seven patients (85.7%) in cluster A and 6 of 19 (31.6%) in cluster B retained functioning pancreas allograft. CD20/MS4A1 mRNA and protein, in addition to CD 3 protein, were detected in four specimens in cluster B. Three of those four pancreas transplants were subsequently lost. Our study demonstrates the potential association of gene expression with clinical outcome of pancreas transplants and justifies further studies in an independent cohort.
KW - Adult
KW - Biopsy
KW - Cluster Analysis
KW - Feasibility Studies
KW - Gene Expression Profiling
KW - Graft Rejection
KW - Graft Survival
KW - Humans
KW - Middle Aged
KW - Oligonucleotide Array Sequence Analysis
KW - Pancreas
KW - Pancreas Transplantation
KW - Pilot Projects
KW - Polymerase Chain Reaction
KW - Predictive Value of Tests
KW - RNA, Messenger
KW - Retrospective Studies
KW - Time Factors
KW - Transplantation, Homologous
KW - Treatment Outcome
U2 - 10.1097/TP.0b013e31818c8fbf
DO - 10.1097/TP.0b013e31818c8fbf
M3 - SCORING: Journal article
C2 - 19155976
VL - 87
SP - 222
EP - 226
JO - TRANSPLANTATION
JF - TRANSPLANTATION
SN - 0041-1337
IS - 2
ER -